In vitro Antidiabetic, Antioxidant and Anti-lipid peroxidative Activities of Corn Steep Liquor Extracts of Curculigo pilosa and its Solvent Fractions

Abstract

ABSTRACTThe in vitro antioxidant, antidiabetic, and antiperoxidative activities of corn steep liquor extract of Curculigo pilosa, and its solvent fractions in n-butanol and methanol were evaluated. The antioxidant ability was evaluated by total phenolic content (TPC), total flavonoid content (TPC), total antioxidant capacity (TAC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability and reducing capacity, antidiabetic activity by ability to inhibit α-amylase and α-glucosidase enzymes though antiperoxidative activity was evaluated by the ability to inhibit Fe2+-induced lipid peroxidation in pancreas. The results showed that the crude extract (CRD) had the highest TPC (63.39 mg GAE 100 g−1), TFC (48.70 mg QUE 100 g−1), and TAC (24.72 mg AAE 100 g−1), which culminated to higher DPPH scavenging effect, reducing power, and lipid peroxidation inhibition in pancreas homogenate. CRD demonstrated the highest ability to inhibit α-amylase and α-glucosidase enzymes.