High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping.

Max R Schroeder,Dhwani Batra,Peyton Smith,Vladimir N Loparev,Lisley Garcia-Toledo,Mili Sheth,Rebecca L Lindsey,Lori A Rowe,Phalasy Juieng,Kristen Knipe

doi:10.1128/genomea.01384-17

Abstract

ABSTRACTEscherichia spp., including E. albertii and E. coli, Shigella dysenteriae, and S. flexneri are causative agents of foodborne disease. We report here reference-level whole-genome sequences of E. albertii (2014C-4356), E. coli (2011C-4315 and 2012C-4431), S. dysenteriae (BU53M1), and S. flexneri (94-3007 and 71-2783).

Highlights

Escherichia spp., including E. albertii and E. coli, Shigella dysenteriae, and S. flexneri are causative agents of foodborne disease
Generated contigs were corrected by aligning Illumina reads using Pilon version 1.21 (3)
Whole-genome optical maps were created using NcoI or AflII digestion with the Argus platform (OpGen, Gaithersburg, MA, USA), and the genomic sequences were verified using corresponding in silico restriction enzyme maps

Summary

Introduction

Escherichia spp., including E. albertii and E. coli, Shigella dysenteriae, and S. flexneri are causative agents of foodborne disease. E. coli E. coli S. dysenteriae S. flexneri S. flexneria Associated plasmid size (bp) (GenBank accession no.)

Results

Conclusion