Abstract
Bradyrhizobiumsp. Tv2a.2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing root nodule of Tachigali versicolor collected in Barro Colorado Island of Panama. Here we describe the features of Bradyrhizobiumsp. Tv2a.2, together with high-quality permanent draft genome sequence information and annotation. The 8,496,279 bp high-quality draft genome is arranged in 87 scaffolds of 87 contigs, contains 8,109 protein-coding genes and 72 RNA-only encoding genes. This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
Highlights
Legumes engage in nitrogen-fixation symbioses with bacterial partners from at least 13 genera of Proteobacteria [1,2,3,4]
Despite the high extent of phylogenetic diversity of root nodule bacteria, the very broad distribution of one particular genus (Bradyrhizobium) across host legume clades suggests that bacteria in this genus may have been the first legume symbionts [5]
Additional gene prediction analysis and manual functional annotation was performed within the Integrated Microbial Genomes-Expert Review (IMGER) system [38] developed by the Joint Genome Institute, Walnut Creek, CA, USA
Summary
Legumes engage in nitrogen-fixation symbioses with bacterial partners from at least 13 genera of Proteobacteria [1,2,3,4]. We report the genome sequence of one such organism, Bradyrhizobium strain Tv2a.2. Strain Tv2a.2 was sampled in 1997 from the tree Tachigali versicolor on Barro Colorado Island, Panama, a biological preserve with an old-growth moist tropical forest [10].
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