High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India.

H S Gehlot ,Sonam Rathi,Ahmed M Al-Hejin,Wayne Reeve,Mohammed N Baeshen,Manoj Pillay,T B K Reddy,Nisha Tak,Ravi Tiwari,Natalia Ivanova,Julie Ardley,Rui Tian,Amrita Pati,Victor Markowitz,Tanja Woyke,Neetu Poonar,Wan Adnawani,Rekha Seshadri,Nikos C Kyrpides ,Raju Ram Meghwal

doi:10.1186/s40793-016-0157-7

Abstract

Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-016-0157-7) contains supplementary material, which is available to authorized users.

Highlights

The genus Prosopis comprises about 44 species that are widely distributed in the world’s semi-arid regions, mostly in North and South America with a few species found in Africa and south west Asia [2,3,4]
P. cineraria is a good candidate for rehabilitation of dry, marginal or degraded lands of low fertility and/or high salinity
WSM1721, two strains isolated from perennial legumes growing in arid climates and alkaline soils in India and Australia, respectively [21, 42]

Summary

Introduction

The genus Prosopis (family Leguminosae, sub-family Mimosoideae [1]) comprises about 44 species that are widely distributed in the world’s semi-arid regions, mostly in North and South America with a few species found in Africa and south west Asia [2,3,4]. PC2 effectively nodulates the Central American mimosoid tree Leucaena leucocephala (Lam.) de Wit. PC2 appears to be a relatively promiscuous strain that has potential to be used as an inoculant for crop legumes species such as Vigna radiata (L.) Wilczek and V. unguiculata (L.) Walp. Strain PC2 on a range of selected hosts are summarised in Additional file 2: Table S2 Genome project history This organism was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the Genomic Encyclopedia of Bacteria and Archaea, The Root Nodulating Bacteria chapter project at the U.S Department of Energy, Joint Genome Institute. The majority of protein-coding genes (76.34 %) were assigned a putative function whilst the remaining genes were annotated as

6.71 Function unknown

Conclusion

11. Felker P

54. Kuykendall LD

Findings

59. Biological Agents