Abstract
The verification of the theoretical equations for the elution characteristics in stepwise and combined two-step isocratic—gradient elution chromatography is described for four N, N-dimethyl- p-aminobenzeneazobenzoyl amides chromatographed on Porasil A using a binary mobile phase consisting of ethyl acetate and cyclohexane. Good agreement between the experimental and calculated values was found. The resolutions is isocratic, simple gradient, stepwise and combined two-step isocratic—gradient elution are compared and the results achieved in the gradient elution chromatography of some, N,N-dimethyl- p-aminobenzeneazobenzoyl amides and esters on silica are presented.
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