Abstract
Theoretical assumptions concerning the influence of the composition of binary mobile phases on capacity ratios in adsorption chromatography were verified using model experiments with chromatography of four N,N-dimethyl- p-aminobenzeneazobenzoylamides on columns packed with silica (Porasil A) in binary mobile phases composed of ethyl acetate and cyclohexane. In this system, good agreement was found between the experimental values of the elution characteristics (retention volume, peak width, resolution and number of theoretical plates) in isocratic and gradient elution chromatography and the values calculated according to the theoretical relationships derived in Parts I and II of this series. Twenty different gradient functions (linear, convex and concave) were tested and the agreement between experiment and theory was found to be satisfactory in all cases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
