Abstract
Theoretical assumptions concerning the influence of the composition of binary mobile phases on capacity ratios in adsorption chromatography have been verified for binary mobile phases composed of ethanol-γ-pentane; n-propanol- n-heptane; dioxane- n-heptane and chloroform- n-heptane. Calculated and experimental values of retention characteristics in gradient elution chromatography have also been compared for these systems. The agreement between experiment and theory was satisfactory, just as it was for a mobile phase composed of cyclohexane and ethyl acetate (as described in Part III of this series). Practical aspects of the solvent systems tested as the mobile phase in gradient elution chromatography are discussed, and the relationship of plate number to composition of mobile phase is considered.
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