Abstract
The complete genome sequencing and analysis of a classical swine fever virus (CSFV) detected in a porcine kidney cell line revealed a close relationship with genotype 1.1 viruses circulating in India and China. The presence of consecutive T insertions in the 3′ untranslated region (UTR), as seen in vaccine strains of CSFV, suggested some degree of attenuation.
Highlights
The complete genome sequencing and analysis of a classical swine fever virus (CSFV) detected in a porcine kidney cell line revealed a close relationship with genotype 1.1 viruses circulating in India and China
We report here the complete genome sequence of CSFV (CSFVPK15C-NG79-11), detected as an adventitious pestivirus while screening cell lines and tissue culture reagents before their use in the laboratory
The whole genome of CSFV-PK15CNG79-11 was amplified as overlapping fragments by reverse transcription-PCR (RT-PCR) and directly sequenced in both directions on an ABI Prism 3500xL DNA sequencer
Summary
The complete genome sequencing and analysis of a classical swine fever virus (CSFV) detected in a porcine kidney cell line revealed a close relationship with genotype 1.1 viruses circulating in India and China. The causative agent, CSF virus (CSFV), belongs to the genus Pestivirus within the family Flaviviridae. The CSFV genome is a single positive-stranded RNA of approximately 12.3 kb containing an ~11.7-kb-long single open reading frame (ORF) flanked by the 5= and 3= untranslated regions (UTRs). The UTRs contain signals for viral replication, transcription, and translation.
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