Abstract
Here, we report the complete genome sequence of a bovine viral diarrhea virus (BVDV) belonging to genotype 1d, strain 10JJ-SKR, which was isolated from cattle. The complete genome is 12,267 nucleotides (nt) in length, with a single large open reading frame. This is the first report of a BVDV belonging to genotype 1d and will enable further study of the molecular and epidemiological characteristics of this virus.
Highlights
We report the complete genome sequence of a bovine viral diarrhea virus (BVDV) belonging to genotype 1d, strain 10JJSKR, which was isolated from cattle
BVDV has been classified into two species, BVDV-1 and BVDV-2 [4]
Total viral RNA was extracted from infected Madin-Darby bovine kidney (MDBK) epithelial cells using an RNeasy minikit (Qiagen; catalog no. 74104). cDNA was obtained by reverse transcription-PCR with Superscript III reverse transcriptase (Invitrogen)
Summary
We report the complete genome sequence of a bovine viral diarrhea virus (BVDV) belonging to genotype 1d, strain 10JJSKR, which was isolated from cattle. Bovine viral diarrhea virus (BVDV) is common in cattle and causes significant economic losses to the livestock industry [1,2,3]. BVDV, together with classical swine fever virus and border disease virus, belongs to the genus Pestivirus within the family Flaviviridae. The complete genome sequences of BVDV-1a, -1b, -1e, -1m, -2a, and -2b have been reported.
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