Genome Sequence of Bacillus velezensis S141, a New Strain of Plant Growth-Promoting Rhizobacterium Isolated from Soybean Rhizosphere.

Surachat Sibponkrung,Neung Teaumroong,Takahiko Kondo,Ken-Ichi Yoshida,Nantakorn Boonkerd,Panlada Tittabutr,Kosei Tanaka

doi:10.1128/genomea.01312-17

Surachat Sibponkrung, Neung Teaumroong + Show 5 more

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https://doi.org/10.1128/genomea.01312-17

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Abstract

ABSTRACTBacillus velezensis strain S141 is a plant growth-promoting rhizobacterium isolated from soybean (Glycine max) rhizosphere that enhances soybean growth, nodulation, and N2 fixation efficiency by coinoculation with Bradyrhizobium diazoefficiens USDA110. The S141 genome was identified to comprise a 3,974,582-bp-long circular DNA sequence encoding at least 3,817 proteins.

Highlights

Bacillus velezensis strain S141 is a plant growth-promoting rhizobacterium isolated from soybean (Glycine max) rhizosphere that enhances soybean growth, nodulation, and N2 fixation efficiency by coinoculation with Bradyrhizobium diazoefficiens USDA110
Strain NAU-B3 was recently reclassified as B. velezensis [7], and we decided to name strain S141 B. velezensis S141
Its genome comprises at least 3,817 protein-coding genes. Compared with another Plant growth-promoting rhizobacteria (PGPR) Bacillus strain, B. velezensis FZB42 (B. amyloliquefaciens subsp. plantarum FZB42) [7], strain S141 contains putative genes involved in indole-3acetic acid (IAA) production [8], including ipdC and dhaS encoding indole-3-pyruvate decarboxylase and indole-3-acetaldehyde dehydrogenase, respectively, to synthesize IAA from indole-3-pyruvic acid; putative iaaH encoding indole-3-acetamide hydrolase to synthesize IAA from indole-3-acetamide; and yhcX encoding nitrilase to synthesize IAA from indole-3-acetonitrile

Summary

Introduction

Bacillus velezensis strain S141 is a plant growth-promoting rhizobacterium isolated from soybean (Glycine max) rhizosphere that enhances soybean growth, nodulation, and N2 fixation efficiency by coinoculation with Bradyrhizobium diazoefficiens USDA110. B. velezensis S141 genomic DNA was prepared using the Wizard Genomic DNA purification kit (Promega, USA), and a genomic DNA library was constructed using the NEBNext ultra DNA library prep kit for Illumina (New England BioLabs Inc., USA) according to the manufacturer’s protocol. B. velezensis S141 genome sequencing was performed on an Illumina MiSeq platform.

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