Abstract
ABSTRACTHerein, we report the genome sequence of a Clostridium difficile strain isolated from the feces of antibiotic-treated C57BL/6 mice. We have named this strain, which differs considerably from those of the previously sequenced C. difficile strains, LEM1.
Highlights
We report the genome sequence of a Clostridium difficile strain isolated from the feces of antibiotic-treated C57BL/6 mice
Fecal samples tested positive for C. difficile upon antibiotherapy were plated onto taurocholate-cefoxitin-cycloserine-fructose agar [7, 8] and incubated anaerobically (48 h, 37°C) [9]
Genomic DNA was extracted from a pure spore preparation of C. difficile LEM1, according to the Pacific Biosciences phenol-chloroform protocol (PacBio), with the following modifications prior to DNA extraction: 10 min of incubation with 10% SDS (64°C), 3 min of bead beating with glass beads, and centrifugation (12,000 rpm, 5 min)
Summary
We report the genome sequence of a Clostridium difficile strain isolated from the feces of antibiotic-treated C57BL/6 mice. Fecal samples tested positive for C. difficile upon antibiotherapy were plated onto taurocholate-cefoxitin-cycloserine-fructose agar [7, 8] and incubated anaerobically (48 h, 37°C) [9]. Isolates that exhibited the characteristic C. difficile colony morphologies were further isolated on brain heart infusion agar supplemented with yeast extract and subsequently tested PCR positive for the presence of the tcdB gene (data not shown). C. difficile spores were prepared as described by Edwards et al [10].
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