Abstract
Here, we report the genome sequence of a novel iflavirus strain recovered from the neotropical butterfly Heliconius erato. The coding DNA sequence (CDS) of the iflavirus genome was 8,895 nucleotides in length, encoding a polyprotein that was 2,965 amino acids long.
Highlights
We report the genome sequence of a novel iflavirus strain recovered from the neotropical butterfly Heliconius erato
Heliconius erato is native to Central and South America where a huge diversity of wing phenotypes are expressed across races from different geographic locations [3]
The Trinity-assembled nucleotide contigs were translated into amino acid sequences, and a BLASTp comparison to the NCBI NR protein database [6] revealed the presence of a viral polyprotein
Summary
We report the genome sequence of a novel iflavirus strain recovered from the neotropical butterfly Heliconius erato. Little is known about the viral (and bacterial) assemblages infecting Heliconius butterflies. We sequenced the mRNA of one male and one female of H. erato, obtained from a Costa Rican butterfly farm, Suministros Entomológicos Costarricenses, S.A. RNA was extracted from two individuals for each of four tissue types (the antenna, mouthparts, head, and legs) using TRIzol (Life Technologies).
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