Abstract

We report here the full mitochondrial genome sequence of Limonius californicus, a species of click beetle that is an agricultural pest in its larval form. The circular genome is 16.5 kb and contains 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes.

Highlights

  • A single specimen of L. californicus was collected on 10 December 2014 from a farmer’s field in Aberdeen, ID, at a 6-inch depth into the soil

  • Control methods for larvae were developed in the 1950s and involved the wide application of environmentally persistent chemicals, such as dichlorodiphenyltrichloroethane (DDT) and ␥-hexachlorocyclohexane (Lindane) [3], both of which were later banned for use and are not used on crops today due to health and environmental risks

  • The specimen species was confirmed on site, and DNA extraction was conducted using cetyltrimethylammonium bromide (CTAB) methodology

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Summary

Introduction

A single specimen of L. californicus was collected on 10 December 2014 from a farmer’s field in Aberdeen, ID, at a 6-inch depth into the soil. Wireworms are the larval stage of click beetles (Coleoptera: Elateridae), of which several species are serious pests due to their damage to the seedlings, roots, and stem tissues of economically important crops [1, 2]. Control methods for larvae were developed in the 1950s and involved the wide application of environmentally persistent chemicals, such as dichlorodiphenyltrichloroethane (DDT) and ␥-hexachlorocyclohexane (Lindane) [3], both of which were later banned for use and are not used on crops today due to health and environmental risks.

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