Abstract
Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit. Long-read sequencing was employed to generate finished genomes for two isolates (each with one chromosome and two plasmids) from symptomatic plants in northern California.
Highlights
Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit
Xanthomonas fragariae causes angular leaf spot (ALS) of strawberry (Fragaria ϫ ananassa) and is an international quarantine pathogen [1, 2]. This species of Gram-negative bacteria colonizes strawberry foliage and enters the leaves through wounds or stomata [3], where it may remain quiescent before initiating growth and the profuse production of exopolysaccharides which is associated with typical water-soaked leaf lesions [2, 3]
Genomic DNA was extracted from cells growing exponentially in liquid Wilbrinks-N [5] using a DNeasy blood and tissue kit (Qiagen, Valencia, CA)
Summary
Xanthomonas fragariae is a foliar pathogen of strawberry that is of significant concern to nursery production of strawberry transplants and field production of strawberry fruit. Xanthomonas fragariae causes angular leaf spot (ALS) of strawberry (Fragaria ϫ ananassa) and is an international quarantine pathogen [1, 2]. A draft genome of a Belgian X. fragariae strain (LMG 25863) was published previously [4].
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