Abstract
Sequence type 258 (ST258) is the most widespread multidrug resistant (MDR) Klebsiella pneumoniae strain worldwide. Here, we report the draft genome sequences of two colistin-resistant MDR K. pneumoniae ST258 clinical strains isolated from hospital patients in Italy. These strains are resistant to β-lactams, cephalosporins, fluoroquinolones, aminoglycosides, macrolides, tetracyclines, carbapenems, and colistin.
Highlights
Mid- to late logarithmic growth phase cells of Vibrio parahaemolyticus grown in tryptic soy broth (TSB) containing 0.5, 3.0, and 7.5% NaCl were heated for 8 min at 45°C in 0.1 M phosphate buffer containing 3% NaCl
NaCl was greatest for unheated cells that had been grown in 7.5% NaCl-TSB; cells grown in 0.5% NaCl-TSB formed a greater number of colonies on 1.0% NaCl-thiosulfate-citrate-bile salts-sucrose agar (TCBS)
Cells of V. parahaemolyticus originally grown in 0.5% NaCl-TSB showed a somewhat different pattern of tolerance to NaCl in the TCBS recovery medium than did cells grown in 3.0 and 7.5% NaCl-TSB
Summary
Mid- to late exponential cells grown at 30°C in various concentrations of NaCl-TSB (0.5, 3.0, and 7.5%) were thermally treated by transferring 10 ml of culture to a 500-ml Erlenmeyer flask containing 190 ml of 3% NaCl (wt/ vol) buffered at pH 7.3 with 0.1 M potassium phosphate (salt buffer) equilibrated at 45°C in a New Brunswick AquaTherm waterbath shaker TCBS (containing 1% NaCl) was supplemented with 0.25, 0.50, 0.75, and 1.0% (anhydrous wt/vol) of MgCl2 or KCI, or with an additional 1.0% NaCl. V. parahaemolyticus was heat-stressed in salt buffer as described above, serially diluted in salt buffer, and surface-plated (0.1 ml) on the TCBS test media. Data presented represent averages of a minimum of two replications run in duplicate
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