Abstract
ABSTRACTStreptococcus thermophilus, a very important dairy species, is constantly threatened by phage infection. We report the genome sequences of three S. thermophilus bacteriophages isolated from a dairy environment in the Veneto region of Italy. These sequences will be used for the development of new strategies to detect and control phages in dairy environments.
Highlights
Streptococcus thermophilus is a low-GC Gram-positive bacterium considered the second most important dairy species [1] and is commonly used to produce cheese and yogurts [2, 3]
We report the genome sequences of three S. thermophilus bacteriophages isolated from a dairy environment in Northeast Italy, vB_SthS_VA214, vB_SthS_VA460, and the partial genome sequence of vB_SthS_VA698 (VA214, VA460, and VA698, respectively)
Coverage values obtained for VA214, VA460, and VA698 were approximately 367, 308, and 122-fold, respectively
Summary
Streptococcus thermophilus is a low-GC Gram-positive bacterium considered the second most important dairy species [1] and is commonly used to produce cheese and yogurts [2, 3]. We report the genome sequences of three S. thermophilus bacteriophages isolated from a dairy environment in Northeast Italy, vB_SthS_VA214, vB_SthS_VA460, and the partial genome sequence of vB_SthS_VA698 (VA214, VA460, and VA698, respectively). Bacteriophages were concentrated and purified using polyethylene glycol 8,000, and their genomic DNA was extracted following the method described by Binetti et al [11].
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