Abstract
ABSTRACTEscherichia coli is involved in the pathogenicity of metritis in cows. We report here the genome sequences of E. coli strains isolated at calving from the uterus, vagina, vulva, and rectoanal junction of a dairy cow that later developed metritis. The genomic similarities will give an insight into phylogenetic relationships among strains.
Highlights
Escherichia coli is involved in the pathogenicity of metritis in cows
A fimH gene phylogenetic tree obtained from the neighbor-joining method indicated the presence of two groups of E. coli strains; only one group included strains found in all body habitats
The group having E. coli strains from all body habitats was chosen for this study, and representative isolates from the uterus (KG-8), vagina (KG-10), vulva (KG-24), and rectoanal junction (KG-16) were selected for wholegenome sequencing
Summary
Escherichia coli is involved in the pathogenicity of metritis in cows. We report here the genome sequences of E. coli strains isolated at calving from the uterus, vagina, vulva, and rectoanal junction of a dairy cow that later developed metritis. Swabs from the uterus, vagina, vulva, and rectoanal junction were taken from a dairy cow (ID 8749) within 30 min after calving. The swabs were resuspended in 1 ml of Luria-Bertani broth (Sigma-Aldrich), and 200 l of diluted sample was cultured overnight on CHROMagar E. coli (CHROMagar).
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