Abstract
ABSTRACTTwo strains of Bacillus, B. cereus E41 and B. anthracis F34, were isolated from a salt lake in Aïn M’lila-Oum El Bouaghi, eastern Algeria, and Ain Baida-Ouargla, southern Algeria, respectively. Their genomes display genes for the production of several bioactive secondary metabolites, including polyhydroxyalkanoate, iron siderophores, lipopeptides, and bacteriocins.
Highlights
Two strains of Bacillus, B. cereus E41 and B. anthracis F34, were isolated from a salt lake in Aïn M’lila-Oum El Bouaghi, eastern Algeria, and Ain Baida-Ouargla, southern Algeria, respectively
Genomic DNA was extracted from single-colony isolates using the DNeasy blood and tissue kit (Qiagen), according to the manufacturer’s protocol
Species identities were determined by an average nucleotide identity (ANI) of Ͼ97% using JSpecies version 1.2.1 (2) with previously sequenced genomes in the GenBank database
Summary
Two strains of Bacillus, B. cereus E41 and B. anthracis F34, were isolated from a salt lake in Aïn M’lila-Oum El Bouaghi, eastern Algeria, and Ain Baida-Ouargla, southern Algeria, respectively. Genomic DNA was extracted from single-colony isolates using the DNeasy blood and tissue kit (Qiagen), according to the manufacturer’s protocol. Sequencing libraries from the genomic DNA extracts were prepared using the Nextera XT DNA library preparation kit (Illumina). Whole-genome sequencing was performed using the MiSeq reagent kit version 2 (2 ϫ 250 cycles) and MiSeq sequencing technology (Illumina), generating 150-bp paired-end reads.
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