Draft Genome Sequence of Pseudomonas stutzeri Strain KMS 55, an Endophytic Diazotroph Isolated from Rice Roots.

Kannepalli Annapurna,Surendra K Chikara,Venkadasamy Govindasamy,Karivaradharajan Swarnalakshmi,Meenakshi Sharma,Yuvika Rajrana,Sangeeta Paul,Arpita Ghosh

doi:10.1128/genomea.00972-17

Abstract

ABSTRACTPseudomonas stutzeri strain KMS 55 (MTCC 12703) is an isolate from the root tissues of rice (Oryza sativa L.) that displays a high biological nitrogen fixation ability. Here, we report the complete genome sequence of this strain, which contains 4,637,820 bp, 4,289 protein-coding genes, 5,006 promoter sequences, 62 tRNAs, a single copy of 5S-16S-23S rRNA, and a genome average GC content of 51.18%. Analysis of the ~4.64-Mb genome sequence will give support to increased understanding of the genetic determinants of host range, endophytic colonization behavior, endophytic nitrogen fixation, and other plant-beneficial roles of Pseudomonas stutzeri.

Highlights

Pseudomonas stutzeri strain KMS 55 (MTCC 12703) is an isolate from the root tissues of rice (Oryza sativa L.) that displays a high biological nitrogen fixation ability
P. stutzeri has been reported as a ubiquitous soil-dwelling, genetically diverse bacterium with denitrifying capabilities [4, 5]
Genome sequencing was performed with pairedend sequencing libraries using a TruSeq standard Nano DNA library prep kit for Illumina at Eurofins Genomics India Pvt

Summary

Introduction

Pseudomonas stutzeri strain KMS 55 (MTCC 12703) is an isolate from the root tissues of rice (Oryza sativa L.) that displays a high biological nitrogen fixation ability. This isolate was named Pseudomonas stutzeri strain KMS 55 (MTCC 12703), and whole-genome sequencing was performed. Genomic DNA from the exponential growth cultures (1 mL, optical density at 600 nm [OD600] 0.5) of P. stutzeri strain KMS 55 was extracted using the NucleoSpin DNA extraction kit (NucleoSpin, Germany).

Results

Conclusion