Abstract
ABSTRACTThe probiotic bacterium Lactococcus garvieae A1, isolated from soil, is interesting for biomining applications. Here, we report the draft genome sequence and annotation of this strain, with a focus on metal transporter enzymes.
Highlights
The probiotic bacterium Lactococcus garvieae A1, isolated from soil, is interesting for biomining applications
The reads were assembled into 25 contigs, with a median coverage of 901-fold, which resulted in a genome size of 2.04 Mb with a GϩC content of 38.0%
Genome analysis revealed that L. garvieae A1 contained several genes encoding an ABC transporter for metal ions; lead, cadmium, zinc, and mercury-transporting ATPases (EC 3.6.3.x); and cation-transporting ATPases, which can all be attributed to the biomining activities, in addition to the acidic fermentation metabolism
Summary
The probiotic bacterium Lactococcus garvieae A1, isolated from soil, is interesting for biomining applications. Genomic DNA of strain A1 was isolated using a Genomic DNA isolation kit (Invitrogen), according to the manufacturer’s protocol. The sequencing was performed using an Illumina HiSeq 2000 sequencing platform (Illumina, Hayward, CA, USA) at Macrogen, Inc. A total of 18,275,986 paired-end reads were obtained, each 100 bp long and 250 bp apart.
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