Abstract
Pseudomonas putida SQ1 was isolated for its ability to utilize the plant sugar sulfoquinovose (6-deoxy-6-sulfoglucose) for growth, in order to define its SQ-degradation pathway and the enzymes and genes involved. Here we describe the features of the organism, together with its draft genome sequence and annotation. The draft genome comprises 5,328,888 bp and is predicted to encode 5,824 protein-coding genes; the overall G + C content is 61.58 %. The genome annotation is being used for identification of proteins that might be involved in SQ degradation by peptide fingerprinting-mass spectrometry.
Highlights
Pseudomonas putida strain SQ1 belongs to the family of Pseudomonadaceae in the class of Gammaproteobacteria
There are more than 30 genome sequences of P. putida strains available (e.g., 12 complete and 24 draft genomes in National Center for Biotechnology Information (NCBI); January 2015), including the complete genome sequence of type strain NBRC 14164T [5]
P. putida strain SQ1 was isolated for its ability to utilize the sulfonated plant sugar sulfoquinovose (6-deoxy6-sulfoglucose) as a sole source of carbon and energy for growth, and was enriched from a sample of littoral
Summary
Pseudomonas putida strain SQ1 belongs to the family of Pseudomonadaceae in the class of Gammaproteobacteria. A draft genome sequence of strain SQ1 has been established and annotated in the IMG pipeline, and the annotation has been transferred to a proteomics (Mascot) database for peptide fingerprinting-mass spectrometry: in our present (unpublished) work, the database is used to identify enzymes and genes that are induced during growth with SQ, e.g. in comparison to cells grown with glucose, by two-dimensional protein gel electrophoresis.
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