Abstract
We report the 5.101-Mbp high-quality draft assembly of the Escherichia coli strain ATCC 23506 (serovar O10:K5:H4, also known as NCDC Bi 8337-41) genome. This uropathogenic strain, commonly referred to as E. coli K5, produces N-acetyl heparosan, a glycosaminoglycan-like capsular polysaccharide and precursor to the anticoagulant pharmaceutical heparin. Metabolic reconstruction of this genome will enable the prediction of gene deletions and overexpressions that lead to increased heparosan production.
Highlights
We report the 5.101-Mbp high-quality draft assembly of the Escherichia coli strain ATCC 23506 genome
The K5 capsule is composed of N-acetyl heparosan, a group II capsular polysaccharide (CPS) consisting of a repeating [¡4) -D-glucuronic acid (GlcA) (1¡4) N-acetyl-␣-Dglucosamine (GlcNAc) (1¡]n disaccharide unit (1)
The gene cluster encoding the enzymes required for the biosynthesis of K5 CPS has been characterized elsewhere, annotation of the whole genome sequence will lend further insight into the molecular mechanisms of capsular polysaccharide biosynthesis and transport
Summary
We report the 5.101-Mbp high-quality draft assembly of the Escherichia coli strain ATCC 23506 (serovar O10:K5:H4, known as NCDC Bi 8337-41) genome. Escherichia coli is the most well-characterized organism commonly utilized in metabolic engineering research. The K5 capsule is composed of N-acetyl heparosan, a group II capsular polysaccharide (CPS) consisting of a repeating [¡4) -D-glucuronic acid (GlcA) (1¡4) N-acetyl-␣-Dglucosamine (GlcNAc) (1¡]n disaccharide unit (1).
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