Abstract
The near-complete genomes of two picobirnaviruses (PBVs) in diarrheal stool samples, human picobirnaviruses D and E (HuPBV-D and -E), were genetically characterized. Their RNA-dependent RNA polymerase (RdRp) protein sequences had <66% identities to known PBVs. Due to a single nucleotide insertion, the open reading frame 2 (ORF2) in segment 1 of HuPBV-D was interrupted by a stop codon. A small stem-loop structure overlying the stop codon may result in translational readthrough into the rest of ORF2.
Highlights
The near-complete genomes of two picobirnaviruses (PBVs) in diarrheal stool samples, human picobirnaviruses D and E (HuPBV-D and -E), were genetically characterized
Fewer than 15 segment 1 sequences have been reported and only one fully characterized human PBV genome sequence has been deposited in GenBank
Two divergent PBVs were identified in samples from two individuals from separate outbreaks, and their presence was confirmed by nucleic acid reextraction, reverse transcription-PCR (RT-PCR), and Sanger sequencing
Summary
The near-complete genomes of two picobirnaviruses (PBVs) in diarrheal stool samples, human picobirnaviruses D and E (HuPBV-D and -E), were genetically characterized. Picobirnaviruses (PBVs) are small nonenveloped viruses with a genome consisting of two double-stranded RNA segments first identified in rats in 1988 [1] and partly sequenced first from rabbits in 1999 and from humans in 2000 [2, 3]. The first complete genome was described in 2005 from a human fecal sample [4]. PBVs have been reported in fecal and respiratory samples in humans, other mammals, reptiles, and birds [5,6,7,8,9].
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