Abstract
ObjectiveOrganophosphorus (OP) pesticides are considered hazardous substances because of their high toxicity to nontarget species and their persistence in the environment and agricultural products. Therefore, it is important to develop a rapid, sensitive, and economical method for detecting OP pesticides and their residues in food and the environment.MethodsA broad, selective monoclonal antibody (MAb) for organophosphorus pesticides was produced. Based on the MAb, an enzyme linked immunosorbent assay (ELISA) and an immunochromatography assay (ICA) for detecting OP pesticides in different agricultural products were developed using a binding inhibition format on microtiter plates and a membrane strip, respectively.ResultsUnder the optimized conditions, the IC50 values of the ELISA ranged from 3.7 to 162.2 ng mL–1 for the 8 OP pesticides. The matrix interferences of Apple, Chinese cabbage, and greengrocery were removed by 40-fold dilution, the recoveries from spiked samples ranged from 79.1% to 118.1%. The IC50 values of ICA for the 8 OP pesticides ranged from 11.8 to 470.4 ng mL−1. The matrix interference was removed from the Chinese cabbage and Apple samples with 5-fold dilution, and the interference was removed from the greengrocery samples with 20-fold dilution. The recoveries from the spiked samples ranged between 70.6 and 131.9%. The established ELISA and ICA were specific selectivity for the 8 OP pesticides.ConclusionsThe established ELISA is a sensitive screening method for the detection of OP pesticides, but the ELISA detection method depends on a laboratory platform and requires a relative long assay time and several steps operation. The established ICA is very useful as a screening method for the quantitative, semi-quantitative or qualitative detection of OP pesticides in agricultural products, and it has advantages over ELISA methods with regard to factors such as the testing procedure, testing time, and matrix interferences, among others.
Highlights
Organophosphorus (OP) pesticides are widely used in agriculture for sucking and biting insect pest control, including fruit flies, stem borers, mosquitoes, and Eurygaster cereal bugs
The enzyme linked immunosorbent assay (ELISA) detection method depends on a laboratory platform and requires a relative long assay time
Production of MAb A hybridoma cell line (C8/D3) that stably produced a monoclonal antibody (MAb) against parathion-methyl, parathion, and fenitrothion was selected from the indirect competitive ELISA
Summary
Organophosphorus (OP) pesticides are widely used in agriculture for sucking and biting insect pest control, including fruit flies, stem borers, mosquitoes, and Eurygaster cereal bugs. OP pesticides are considered hazardous substances because of their high toxicity to nontarget species and their persistence in the environment [1]. It is important to develop a rapid, sensitive, and economical method for detecting OP pesticides and their residues in food and the environment. Instrument-based methods, such as gas chromatography and high-performance liquid chromatography, are the most commonly used techniques for detecting OP pesticides in different samples [3,4,5,6]. These commonly used methods require expensive equipment and are only applicable in laboratory settings. Immunoassays have received considerable attention as a simple, sensitive, cost-effective tool for highthroughput screening analyses in pesticide monitoring programs [7]
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