Abstract
Organophosphorus (OP) pesticides are widely used to control pests because of their high activity. This study described a rapid and sensitive lateral flow immunochromatographic (LFIC) assay based on up-converting nanoparticles (UCNPs) for multi-residue detection of three OP pesticides. The developed assay integrated novel fluorescent material UCNPs labeled with a broad-specific monoclonal antibody. Based on the competitive platform by immobilized antigen in the test zone, the optimized UCNPs-LFIC assay enabled sensitive detection for parathion, parathion-methyl, and fenitrothion with IC50 of 3.44, 3.98, and 12.49 ng/mL (R2 ≥ 0.9776) within 40 min. The detectable ability ranged from 0.98 to 250 ng/mL. There was no cross-reactivity with fenthion, phoxim, isocarbophos, chlorpyrifos, or triazophos, even at a high concentration of 500 ng/mL. Matrix interference from various agricultural products was also studied in food sample detection. In the spiked test, recoveries of the three OP pesticides ranged from 67 to 120% and relative standard deviations were below 19.54%. These results indicated that the proposed strip assay can be an alternative screening tool for rapid detection of the three OP pesticides in food samples.
Highlights
Organophosphorus (OP) pesticides, a class of insecticides, are widely used in pest control for their strong effect and broad spectrum (Roberts and Aaron, 2008; Handford et al, 2015)
We focused on preparing up-converting nanoparticles (UCNPs) fluorescent probes coupled with a broad-specific monoclonal antibody that can recognize parathion, methyl-parathion and fenitrothion simultaneously
The broad-specific monoclonal antibody (mAb) was produced and their similar binding properties against three OP pesticides were proved by surface plasmon resonance-based immunosensor-based immunoassays (Jiao et al, 2018)
Summary
Organophosphorus (OP) pesticides, a class of insecticides, are widely used in pest control for their strong effect and broad spectrum (Roberts and Aaron, 2008; Handford et al, 2015). As an inhibitor of acetylcholinesterase, OP pesticides cause the accumulation of neurotransmitters and dysfunctional neuropathy (Davies et al, 1996; Payne-sturges et al, 2009; Bouchard et al, 2011), which pose a significant threat to human health and food safety. Considering their high toxicology, many counties set strict regulations to defend against hazards from OP pesticides, as parathion and parathion-methyl were banned in the European Union, Australia and China. The potential risk of excessive residue in agriproducts is highlighted (Liang et al, 2012). There is an urgent need to establish highly sensitive analytical methods for large-scale monitoring of OP pesticide residue
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