Abstract
The scrub typhus is an endemic disease from Asia caused by a bacteria called Orientia tsutsugamushi which is characterized by the presence of general discomfort, headache, body aches and muscle pain, fever, rash, and enlarged lymph nodes, similar to symptoms of many other diseases as dengue or rickettsia. In Latin America the presence of O. tsutsugamushi has already been reported; nevertheless, as in México the presence of this bacteria has not been detected, is not considered as a part of molecular diagnosis consequently we decided to identify the presence of clinical cases of scrub typhus in negative patients for dengue and rickettsia. The sample was established by 21 patients from public and private hospitals of Yucatán from January until June of 2017, under suspicion of dengue and rickettsia but serologically negative in these. The DNA was extracted and amplified for the gen STA-56, it showed a band in level between 300-400 pb where there was the positive control. With these results through the PCR exists the possibility of having positive cases of O. tsutsugamushi; however, for its low quality in the visualization, those should be confirmed by genetic sequencing.
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