Complete Genome Sequences of Paenibacillus larvae Phages BN12, Dragolir, Kiel007, Leyra, Likha, Pagassa, PBL1c, and Tadhana.

Jamison K Walker,Donald P Breakwell,Philippos K Tsourkas,Casey Stamereilers,Ashley M Payne,Jordan A Berg,Julianne H Grose,Aziza Dhalai,Douglas W Dingman,Cameron Imahara,Bryan D Merrill,Andy T Ward,Bradley K Knabe,Chris P Fajardo,Kiel Graves,James Mangohig,Josh Monk,Hunter L Hill,Sandra Hope,Kathie Velez,Alicia Salisbury,John Hilton ,Hye-Jung Mun

doi:10.1128/genomea.01602-17

Abstract

ABSTRACTWe present here the complete genomes of eight phages that infect Paenibacillus larvae, the causative agent of American foulbrood in honeybees. Phage PBL1c was originally isolated in 1984 from a P. larvae lysogen, while the remaining phages were isolated in 2014 from bee debris, honeycomb, and lysogens from three states in the USA.

Highlights

We present here the complete genomes of eight phages that infect Paenibacillus larvae, the causative agent of American foulbrood in honeybees
The phages were isolated from bee debris, honeycomb, and lysogens and amplified in P. larvae field isolates
Phage genomes were sequenced in the Brigham Young University (BYU) DNA Sequencing Center using the Illumina HiSeq 2500 platform (Illumina, Hayward, CA, USA) and were assembled using Geneious 8 software (Biomatters Inc., Newark, NJ, USA)

Summary

Introduction

We present here the complete genomes of eight phages that infect Paenibacillus larvae, the causative agent of American foulbrood in honeybees. The phages were isolated from bee debris, honeycomb, and lysogens and amplified in P. larvae field isolates. Phage genomic DNA was isolated from high-titer lysates using Norgen phage DNA isolation kits (Norgen Biotek, Thorold, ON, Canada). Phage genomes were sequenced in the BYU DNA Sequencing Center using the Illumina HiSeq 2500 platform (Illumina, Hayward, CA, USA) and were assembled using Geneious 8 software (Biomatters Inc., Newark, NJ, USA).

Results

Conclusion