Complete Genome Sequence of Rift Valley Fever Virus Strain Lunyo.

Sarah Lumley,Nicholas Johnson,Richard J Ellis,Anthony R Fooks,Denise A Marston,Roger Hewson,Daniel L Horton

doi:10.1128/genomea.00170-16

Abstract

Using next-generation sequencing technologies, the first complete genome sequence of Rift Valley fever virus strain Lunyo is reported here. Originally reported as an attenuated antigenic variant strain from Uganda, genomic sequence analysis shows that Lunyo clusters together with other Ugandan isolates.

Highlights

Using next-generation sequencing technologies, the first complete genome sequence of Rift Valley fever virus strain Lunyo is reported here
Double-stranded cDNA was synthesized from 50 ng of RNA using the cDNA Synthesis System (Roche) in accordance with the manufacturer’s instructions. ds-cDNA was purified using Agencourt AMPure XP system (Beckman Coulter)
The S segment intergenic region nucleotides were resolved by amplicon-based next-generation sequencing using primers binding to terminal regions [11]

Summary

Introduction

Using next-generation sequencing technologies, the first complete genome sequence of Rift Valley fever virus strain Lunyo is reported here. Rift Valley fever virus (RVFV) is a Phlebovirus, in the family Bunyaviridae, and was first isolated in 1930. The virus cycles between its mosquito vector and mammalian hosts. The strain Lunyo was isolated from a pool of Aedes mosquitoes in the Lunyo Forest, Uganda, in 1955 [2].

Results

Conclusion