Abstract
A safe, highly sensitive, specific, and economic method was developed for early detection of RNA and differentiation of Rift Valley fever (RVF) virus strains (isolates) in samples of organs from sick and suspected animals, virus-containing culture materials, and objects of veterinary inspection by means of two-round PCR and restriction analysis of PCR products with the use of a set of the restriction by endonucleases. The method can be included in the scheme of early diagnosis of disease and monitoring the spread of the virus. Restriction analysis allows revealing unique marker sites of amplificates of different RVF virus strains during their study, identification, and certification.
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