Abstract
The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.
Highlights
The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR
The Papaya ringspot virus (PRSV)-resistant genetically modified (GM) papaya has been commercially grown in Hawaii since 1998 and has played the major role in saving the papaya industry from economical demise [3, 5, 7,8,9]
Leaves showing distorted and mosaic symptoms from Huanong No 1 GM papaya were collected for total RNA isolation using the TRIzol reagent (Invitrogen, USA)
Summary
The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. Papaya ringspot virus (PRSV), a filamentous flexuous rod virus (760 to 800 ϫ 12 nm) with a single-stranded positive-sense RNA as its genome [1, 2] belongs to the genus Potyvirus in the family Potyviridae [3]. PRSV infects mainly papaya and cucurbits in the field. The PRSV-resistant genetically modified (GM) papaya has been commercially grown in Hawaii since 1998 and has played the major role in saving the papaya industry from economical demise [3, 5, 7,8,9].
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