Abstract
pAP13 is an 89-kb linear plasmid hosted by Brevibacterium sp. strain Ap13, an actinobacterium isolated from the feces of a flamingo from an extremely high-altitude lake in Argentina. Because of the ecological importance of the genus Brevibacterium, the absolute lack of information concerning Brevibacterium linear plasmids, and the possible ecological significance of this unusual plasmid, pAP13 was completely sequenced, including the inversely oriented termini.
Highlights
PAP13 is an 89-kb linear plasmid hosted by Brevibacterium sp. strain Ap13, an actinobacterium isolated from the feces of a flamingo from an extremely high-altitude lake in Argentina
In 2010, we showed for the first time the presence of a novel class of extrachromosomal elements in this genus, i.e., the linear plasmid pAP13 (4)
Its linearity was demonstrated from pulsed-field gel electrophoresis, and the apparent presence of terminal proteins covalently linked to the 5=-ends was deduced from treatments with exonucleases
Summary
PAP13 is an 89-kb linear plasmid hosted by Brevibacterium sp. strain Ap13, an actinobacterium isolated from the feces of a flamingo from an extremely high-altitude lake in Argentina. Since the first description of the genus Brevibacterium in 1953 by Breed (1), more than 40 species have been described (http: //www.dsmz.de/), including representatives of environmental, biotechnological, and industrial interest, as well as those of clinical significance. Currently only nine sequencing genome projects are running (according to the National Center for Biotechnological Information [NCBI]), and only one complete genome has been reported far, for the species Brevibacterium senegalense (2).
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