Abstract
ABSTRACTThe two linear plasmids pLMA1 (109,112 bp) and pLMA7 (82,075 bp) from Micrococcus strains were isolated from a high-altitude lake in the Argentinean Puna, sequenced, and annotated. These extrachromosomal elements are probably conjugative and harbor genes potentially involved in coping with the harsh conditions in such extreme environments.
Highlights
The two linear plasmids pLMA1 (109,112 bp) and pLMA7 (82,075 bp) from Micrococcus strains were isolated from a high-altitude lake in the Argentinean Puna, sequenced, and annotated
The linear plasmid pLMA1 was sequenced by a combination of Sanger sequencing and 454 pyrosequencing
Annotation was performed by the Integrated Microbial Genomes (IMG) annotation pipeline [6]
Summary
The two linear plasmids pLMA1 (109,112 bp) and pLMA7 (82,075 bp) from Micrococcus strains were isolated from a high-altitude lake in the Argentinean Puna, sequenced, and annotated. The linear plasmid pLMA1 was sequenced by a combination of Sanger sequencing and 454 pyrosequencing. This strategy was previously successfully applied for analysis of cloned fragments of pLMA1 [5]. The 454-shotgun library was constructed and sequenced with the Genome Sequencer FLX system using titanium chemistry as recommended by the manufacturer (454 Life Sciences, Roche Applied Science, Branford, CT).
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