Abstract
We report the complete genome sequence of Mycoplasma ovis strain Michigan. Its single circular chromosome has 702,511 bp and contains 2 copies of the 16S rRNA gene, one corresponding to M. ovis and the other to “Candidatus Mycoplasma haemovis.” All housekeeping genes and the 5S-23S rRNA genes are present in single copies.
Highlights
We report the complete genome sequence of Mycoplasma ovis strain Michigan
After assembly resulting from genome coverages of 1,500ϫ and 1,000ϫ for Illumina HiSeq and Illumina MiSeq, respectively, 3 remaining gaps were closed using conventional PCR, followed by Sanger sequencing in both directions
The complete genome of M. ovis Michigan consists of 702,511 bp in a single circular chromosome, with an average GϩC content of 31.7%
Summary
We report the complete genome sequence of Mycoplasma ovis strain Michigan. Its single circular chromosome has 702,511 bp and contains 2 copies of the 16S rRNA gene, one corresponding to M. ovis and the other to “Candidatus Mycoplasma haemovis.” All housekeeping genes and the 5S-23S rRNA genes are present in single copies. Mycoplasma ovis (Eperythrozoon ovis), a hemoplasma infecting sheep, was first described by Neitz et al in 1934 [1]. A second genetically distinct hemoplasma of sheep and goats was detected by cloning and sequencing of its 16S rRNA genes [8].
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