Abstract
ABSTRACTLactobacillus plantarum 10CH is a bacteriocin-producing potential probiotic lactic acid bacterium (LAB) strain isolated from cheese. Its complete nucleotide sequence shows a single circular chromosome of 3.3 Mb, with a G+C content of 44.51%, a 25-gene plantaricin bacteriocin gene cluster, and the absence of recognized virulence factors.
Highlights
Lactobacillus plantarum 10CH is a bacteriocin-producing potential probiotic lactic acid bacterium (LAB) strain isolated from cheese
The draft genome was mapped against the published reference genome of L. plantarum WCSF1 (GenBank accession number AL935263), which was found to be the closest neighbor by the NCBI server using the CONTIGuator mapping tool [8]
Four prophage loci ranging in size from 26 to 51 kb were identified in the chromosome using the PHAST Web server [11]
Summary
Lactobacillus plantarum 10CH is a bacteriocin-producing potential probiotic lactic acid bacterium (LAB) strain isolated from cheese. Genomic DNA of L. plantarum 10CH was isolated using a GeneJET purification kit and assessed using a NanoDrop ND-1000 spectrophotometer and by electrophoresis. Genome sequencing was performed by Microbes NG (University of Birmingham, UK) using MiSeq and HiSeq 2500 platforms (Illumina, UK). The genome sequencing yielded 968,127 reads, with a median insert size of 489 bases and 123-fold coverage of the genome.
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