Abstract
A complete 39,318-bp genome sequence containing 52 coding sequences has been determined for the Bacillus subtilis temperate phage φ105. In a lysogen, B. subtilis strain 1L32, the φ105 prophage interrupts the radC locus, a part of the competence-induced ComK regulon.
Highlights
A complete 39,318-bp genome sequence containing 52 coding sequences has been determined for the Bacillus subtilis temperate phage 105
The present sequence differs in 68 nucleotides from an unpublished genome sequence in the database (AB016282), correcting 15 probable frameshift errors in the older sequence and locating the phage attachment site for the first time
The 105 prophage interrupts the radC gene in the B. subtilis 1L32 genome at a 10-bp attachment site (TGTTTATACTT)
Summary
A complete 39,318-bp genome sequence containing 52 coding sequences has been determined for the Bacillus subtilis temperate phage 105. Bacteriophage 105, a well-studied temperate phage of Bacillus subtilis [1, 2], is capable of specialized transduction and has been developed into a system for cloning and expressing genes in this host [3, 4]. I describe the complete genome resequencing of 105.
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