Abstract
Objective To investigate the clincial value of ELISA combined with colloidal gold immunochromatography in the detection of anti-cyclic citrullinated peptide (CCP) antibody in patients with rheumatoid arthritis (RA). Methods From October 2014 to October 2016, 120 patients with RA who admitted to Fuyang People's Hospital were selected as RA group, and 120 healthy subjects were selected as the control group.Serum samples of patients were collected and tested for anti-CCP antibody by ELISA and ELISA combined with colloidal gold immunochromatography. Results ELISA method was lower than ELISA combined with colloidal gold immunochromatography test (χ2=3.943, P<0.05). The sensitivity, specificity, positive predictive value, negative predictive value of ELISA combined with colloidal gold immunochromatography assay were 93.33%, 91.67%, 84.85%, 96.49%, respectively, which were higher than single ELISA method (all P<0.05). Conclusion ELISA combined with colloidal gold immunochromatography assay for detection of serum anti-CCP antibody in patients with RA is effective and can obtain high diagnostic sensitivity and specificity, and it is worthy of promotion. Key words: Arthritis, rheumatoid; Enzyme-linked immunosorbent assay; Colloidal gold immunochromatography; serum anti-cyclic citrullinated peptide; Sensitivity and specificity
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