Abstract
ABSTRACTWe report here the complete chloroplast genome sequences of seven strains of the bloom-forming raphidophyte Heterosigma akashiwo. These ~160-kb sequences contain 124 protein-, 6 rRNA-, and 34 tRNA-coding sequences. Notable sequence variations were observed among these seven sequenced and two previously characterized strains.
Highlights
We report here the complete chloroplast genome sequences of seven strains of the bloom-forming raphidophyte Heterosigma akashiwo
As a part of our continuous effort to establish strain-specific markers for H. akashiwo, we sequenced full-length chloroplast DNA of seven H. akashiwo strains with different geographic origins: CCAP934/8 (Puget Sound, WA, USA), EHUSP1 (Bay of Biscay, Spain), CCAP934/4 (Tampa Bay, FL, USA), CCMP2274 (San Francisco Bay, CA, USA), CCMP3374 (East Greenwich, RI, USA), HaFk01 (Fukuoka, Japan), and CCMP1596 (Narragansett, RI, USA)
The libraries were sequenced with the Illumina MiSeq platform, and the obtained paired-end reads were mapped to the previously published sequences, H. akashiwo ptDNA of NIES293 (EU168190) and CCMP452 (EU168191) (16), using Burrows-Wheeler alignment software
Summary
We report here the complete chloroplast genome sequences of seven strains of the bloom-forming raphidophyte Heterosigma akashiwo. As a part of our continuous effort to establish strain-specific markers for H. akashiwo, we sequenced full-length chloroplast DNA (ptDNA) of seven H. akashiwo strains with different geographic origins: CCAP934/8 (Puget Sound, WA, USA), EHUSP1 (Bay of Biscay, Spain), CCAP934/4 (Tampa Bay, FL, USA), CCMP2274 (San Francisco Bay, CA, USA), CCMP3374 (East Greenwich, RI, USA), HaFk01 (Fukuoka, Japan), and CCMP1596 (Narragansett, RI, USA). The libraries were sequenced with the Illumina MiSeq platform, and the obtained paired-end reads were mapped to the previously published sequences, H. akashiwo ptDNA of NIES293 (EU168190) and CCMP452 (EU168191) (16), using Burrows-Wheeler alignment software.
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