Abstract
This chapter discusses the measurement of emission in steady state spectra. The emission spectra are the energy spectra of the photons emitted during relaxation of the excited electronic subsystem to the ground state. The chapter deals with the application range of the emission spectroscopy and the differences with an absorption spectroscopy. It demonstrates how emission spectroscopy is much more sensitive and how a very small amount of substance can be monitored. The chapter discusses the measurement of the emission spectrum which is done through instruments named fluorimeters or spectrofluorimeters. The uses of these two instruments are discussed in detail for optical scheme, use of array detectors, evaluation of measured signal, and spectrum correction. The quantum yield determination by comparison method is also explained in the chapter. The excitation monitoring schemes, used in emission spectroscopy, is also illustrated. The chapter outlines the fluorimeter specifications, and commercial fluorimeters. Finally, the emission of molecular monolayer is also explained.
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