Abstract
Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) is a natural compound, which serves as a protective substance in many halophilic actinobacterial cells. Ectoine biosynthesis genes (ectA, B and C) were PCR amplified from the genomic DNA of deep sea actinobacteria, Nocardiopsis alba NIOT-DSB14. The amplified genes were cloned and nucleotide sequences were determined. Sequencing results revealed that ectA, B and C genes contains 471bp, 1266bp, 378bp long ORF encoding 156, 421 and 125 amino acids, respectively. In silico sequence and phylogenetic analysis of nucleotides and amino acids revealed that the ectA, B and C sequences of Nocardiopsis alba NIOT-DSB14 were conserved in many eubacteria.
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