Abstract
Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) is a natural compound, which serves as a protective substance in many halophilic actinobacterial cells. Ectoine biosynthesis genes (ectA, B and C) were PCR amplified from the genomic DNA of deep sea actinobacteria, Nocardiopsis alba NIOT-DSB14. The amplified genes were cloned and nucleotide sequences were determined. Sequencing results revealed that ectA, B and C genes contains 471 bp, 1266 bp, 378 bp long ORF encoding 156, 421 and 125 amino acids, respectively. In silico sequence and phylogenetic analysis of nucleotides and amino acids revealed that the ectA, B and C sequences of Nocardiopsis alba NIOT-DSB14 were conserved in many eubacteria.
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