Abstract

ABSTRACTA phage-like plasmid isolated from a clinical isolate of Salmonella enterica serovar Derby has strong nucleotide sequence identity to the phage-like plasmids pSTM_phi isolated from Salmonella enterica serovar Typhimurium L495, AnCo1 and AnCo2 from Escherichia coli 243 and Escherichia coli 244, and the virulent Salmonella-specific SSU5 bacteriophage.

Highlights

  • A phage-like plasmid isolated from a clinical isolate of Salmonella enterica serovar Derby has strong nucleotide sequence identity to the phage-like plasmids pSTM_phi isolated from Salmonella enterica serovar Typhimurium L495, AnCo1 and AnCo2 from Escherichia coli 243 and Escherichia coli 244, and the virulent Salmonella-specific SSU5 bacteriophage

  • Derby isolate to determine the nature of the phage-like plasmid

  • This is the 10th documented report of a phage-like plasmid; we suggest that phage-like plasmids may represent a new genus of phages

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Summary

Introduction

A phage-like plasmid isolated from a clinical isolate of Salmonella enterica serovar Derby has strong nucleotide sequence identity to the phage-like plasmids pSTM_phi isolated from Salmonella enterica serovar Typhimurium L495, AnCo1 and AnCo2 from Escherichia coli 243 and Escherichia coli 244, and the virulent Salmonella-specific SSU5 bacteriophage. A clinical isolate of Salmonella enterica serovar Derby was found to contain a phage-like plasmid. We performed whole-genome sequencing of the S. Derby isolate to determine the nature of the phage-like plasmid. Whole-genome sequencing was performed at the EcoGenomics analysis platform (Ibis; Université Laval, Québec, Canada) on an Illumina MiSeq platform with 300-bp paired-end libraries and 30ϫ coverage.

Results
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