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Abstract
ABSTRACTTwo similar phage-like plasmids carrying CTX-M-15 resistance cassettes were identified from two environmental Escherichia coli isolates. They demonstrate strong nucleotide sequence identity to the phage-like plasmid pECOH89 and Salmonella bacteriophage SSU5.
Highlights
Two similar phage-like plasmids carrying CTX-M-15 resistance cassettes were identified from two environmental Escherichia coli isolates
Two environmental Escherichia coli strains isolated from the feces of wildlife closely associated with bovine feedlots in Colorado, USA, were found to contain phage-like plasmids harboring CTX-M-15
We performed whole-genome sequencing of the two E. coli isolates to determine the nature of the phage-like plasmids
Summary
Two similar phage-like plasmids carrying CTX-M-15 resistance cassettes were identified from two environmental Escherichia coli isolates. Two environmental Escherichia coli strains isolated from the feces of wildlife closely associated with bovine feedlots in Colorado, USA, were found to contain phage-like plasmids harboring CTX-M-15. We performed whole-genome sequencing of the two E. coli isolates to determine the nature of the phage-like plasmids. Whole-genome sequencing was performed at the EcoGenomics analysis platform (Ibis; Université Laval, Québec, Canada) on an Illumina MiSeq using 300-bp paired-end libraries with 30ϫ coverage.
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