Zingiber Officinale Roscoe Rhizome Research Articles

HYPOGLYCEMIC ACTIVITY OF TEN MEDICINAL PLANTS EXTRACT IN GLUCOSE INDUCED MICE

Objective: To evaluate and compare the hypoglycemic activity of ten medicinal plantsextract that are used empirically to treat hyperglycemia in Indonesia, in order to determineplant with the most potential as a hypoglycemic agent.Methods: Activity test was conducted in Swiss Webster glucose induced mice with glucosetolerance test. The ethanolic extracts of Mangiferaindica L. leaves, Perseaamericana Mill.leaves, Acoruscalamus L. rhizome, Phyllanthusniruri L. herb, Syzigiumcumini (L.) skeelsbark, Zingiberofficinale Roscoe rhizome, Moringaoleifera Lam. leaves, TamarindusindicaL. seed, Momordicacharantia L. fruits and Azadirachtaindica A. juss leaves, which weregiven orally at the same dose of 300 mg/kg bw. The blood samples wereused for determination of glucose level examination using glucose meter and test strips (AccuChek).Results: The ethanolic extract of Mangiferaindica L. leaves has the best activity in reductionof blood glucose level in mice, followed by Zingiberofficinale Roscoe rhizome,Acoruscalamus L. rhizome, Tamarindusindica L. seed.,MomordicacharantiaL. fruit., Syzigiumcumini (L.) skeels bark, Phyllanthusniruri L. herb., Perseaamericana Mill. leaves, andAzadirachtaindica A. juss leaves.Conclusion: Results of the present study suggest the potential use of Mangiferaindica L.leaves extract in therapy of hyperglycemia.

Data in support of three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes.

This paper describes data related to a research article titled “Three Phase Partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes” (Gagaoua et al., 2015) [1]. Zingibain (EC 3.4.22.67), is a coagulant cysteine protease and a meat tenderizer agent that have been reported to produce satisfactory final products in dairy and meat technology, respectively. Zingibains were exclusively purified using chromatographic techniques with very low yield purification. This paper includes data of the effect of temperature, usual salts and organic solvents on the efficiency of the three phase partitioning (TPP) system. Also it includes data of the kinetic activity characterization of the purified zingibain using TPP purification approach.

Three phase partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes

The present work describes for the first time an elegant non-chromatographic method, the three phase partitioning for the purification and recovery of zingibain, a milk-clotting enzyme, from Zingiber officinale rhizomes. Factors affecting partitioning efficiency such as (NH4)2SO4 saturation, crude extract to t-butanol ratio and pH on zingibain partitioning were investigated. Optimal purification parameters were 50% (NH4)2SO4 saturation with 1.0:1.0 ratio of crude extract:t-butanol at pH 7.0, which gave 14.91 purification fold with 215% recovery of zingibain. The enzyme was found to be exclusively partitioned in the aqueous phase. The enzyme showed a prominent single band on SDS-PAGE. It is a monomeric protein of 33.8kDa and its isoelectric point is 4.38. The enzyme exhibited maximal proteolytic activity at a temperature of 60°C and pH 7.0. It was found to be stable at 40–65°C during 2h. The enzyme was found to be highly stable against numerous metal ions and its activity was enhanced by Ca2+, K+ and Na+. It was completely inhibited by heavy metal ions such as Cu2+ and Hg2+ and partially by Cd+. Zingibain milk-clotting activity (MCA) was found to be highly stable when stored under freezing (−20°C) for 30 days compared at 4°C.

The Effect of Drying Methods on Physicochemical Properties of Nanostructured Zingiber officinale Rosc. (Ginger) Rhizome

Zingiber officinale Roscoe, family of Zingiberaceae is classified as traditional medicinal that globally consumed as spices, food flavouring as well as remedies to treat women during confinement, to treat stomach upset and diarrhoea.The rhizome has been reported to contain abundant bioactive constituents which are mainly polyphenol and flavonoid. Therefore in this research the effect of cabinet drying (60°C) and freeze drying (-40°C) process during preparation of nanostructure Zingiber officinale Roscoe rhizome on particle sizes, surface morphology, FTIR, as well as Total Phenolic Content (TPC) and Total Flavonoid Content(TFC) were compared. Both drying process affect the particle sizes as well as TPC and TFC value. Finer particle size (254.3+ 9.33) and higher TPC (152.54 mg GAE/ g) and TFC (1.42 mgQE/g) were reported for cabinet dried nanostructure Zingiber officinale Rosc. rhizome as compared to freeze dried. The FESEM Photographs revealed that drying processes did affect the surface morphologies of nanostructure Zingiber officinale Rosc rhizome where cabinet dried produced solid spherical particles with a diameter around 100 – 200 nm and some smaller than100nm. Freeze dried consist of many nanoparticles having rod like structure. Both drying process did not significantly affect the presence of active compounds based on FTIR analysis.