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Abstract
This paper describes data related to a research article titled “Three Phase Partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes” (Gagaoua et al., 2015) [1]. Zingibain (EC 3.4.22.67), is a coagulant cysteine protease and a meat tenderizer agent that have been reported to produce satisfactory final products in dairy and meat technology, respectively. Zingibains were exclusively purified using chromatographic techniques with very low yield purification. This paper includes data of the effect of temperature, usual salts and organic solvents on the efficiency of the three phase partitioning (TPP) system. Also it includes data of the kinetic activity characterization of the purified zingibain using TPP purification approach.
Highlights
This paper describes data related to a research article titled “Three Phase Partitioning of zingibain, a milk-clotting enzyme from Zingiber officinale Roscoe rhizomes” (Gagaoua et al, 2015) [1]
We provide data of the optimal parameters for an efficient purification tool, the Three Phase Partitioning system for high purity and recovery yield of zingibain
A table containing the effect of usual salts and organic solvents on partitioning of zingibain by three phase partitioning (TPP) is given (Table 1)
Summary
Data for high purity purification of zingibain using Three Phase Partitioning (TPP) system are given. The protocol and data described provide optimized parameters for TPP tool for an efficient recovery of zingibain from ginger rhizomes. Kinetic characterizations of the purified zingibain are given for the first time. Zingibain (EC 3.4.22.67), is an extensively used protease in food industry for cheese-making or meat tenderization, and its fast and efficient purification is highly required. We provide data of the optimal parameters for an efficient purification tool, the Three Phase Partitioning system for high purity and recovery yield of zingibain. A table containing the effect of usual salts and organic solvents on partitioning of zingibain by TPP is given (Table 1). In addition a flow sheet of the workflow employed to purify zingibain from fresh Zingiber officinale Roscoe rhizomes followed by the Kinetics (Michaelis-Menten and Lineweaver-Burk plot) of the purified zingibain are shared (Figs. 1 and 2)
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