Microorganisms require nutrients to maintain their physiological functions. Changes in the availability of free amino acids affect the expression of virulence factors, the degree of activity of bacteria, their ability to undergo genetic transformation, and the formation of persistent cells. This study demonstrates that bioactive compounds, such as tryptophan, zinc aspartate, arginine, tatipacin, and taurine, have a modulating effect on the metabolism of microorganisms in vitro. The study found that the metabolic activity of microorganisms increased when exposed to concentrations of bioactive compounds ranging from 0.5–50 µg/ml, particularly in the presence of arginine. Additionally, the presence of BAS in the nutrient medium impacted the growth kinetics of microorganisms. Statistically significant changes were observed compared to the control (without the addition of BAS) after 4 hours of incubation and continued until 36 hours of incubation. The study revealed a significant difference between BAS and control during the 16–24 hour incubation period (p<0.001). The modulation of bacterial activity by BAS can be achieved through various targets and interaction points, such as BAS concentration, the unique cellular structure of the microorganism, its metabolism, and the bacterial pathogen's resistance mechanisms. These findings have broad implications, including the potential for future studies to overcome antibiotic resistance in bacteria.