Aging is a complicated process leading to variation in biological and physical traits of living organisms. In this study, we investigate how the nanomechanical properties of live cells such as Young's modulus, and deformation are affected by aging using Atomic Force Microscopy (AFM) at single molecule level. AFM is an outstanding platform to perform high resolution imaging and nanomechanical property characterization of the biomolecules, and live cells. For mice bone marrow-derived cells (BMDC), there weren’t significant effects of aging on the costimulatory molecule expression of the cells or the cytokine secretion for the non-adherent cell population whereas spleen derived dendritic cells were affected by aging. In our study, mice bone marrow-derived adherent cells were used as a model system for the measurement of nanomechanical properties using AFM. The same PFQNM-LC-CAL-A probe with calibrated spring constant of 0.101 N/m and uniform scanning parameters was used for all the experiments on both aged and younger cells in this study in Peak Force QNM mode. Our results showed that average Young's modulus values of older mice cells (48.13± 6.73 kPa) are higher than younger mice cells (35.37±2.34 kPa) and deformation values of older mice cells are lower compared to younger cells. So, unlike non-adherent BMDCs, nanomechanical properties of adherent BMDCs are affected by aging. We intend to perform similar measurements with AFM on cells derived from older and younger population of reptiles like snake and turtles to improve the understanding of aging process.