Yield Losses In Barley Research Articles

Targeted mutations in the GW2.1 gene modulate grain traits and induce yield loss in barley

Grain Width and Weight 2 (GW2) is an E3-ubiquitin ligase-encoding gene that negatively regulates the size and weight of the grain in cereal species. Therefore, disabling GW2 gene activity was suggested for enhancing crop productivity. We show here that CRISPR/Cas-mediated mutagenesis of the barley GW2.1 homologue results in the development of elongated grains and increased protein content. At the same time, GW2.1 loss of function induces a significant grain yield deficit caused by reduced spike numbers and low grain setting. We also show that the converse effect caused by GW2.1 absence on crop yield and protein content is largely independent of cultivation conditions. These findings indicate that the barley GW2.1 gene is necessary for the optimization between yield and grain traits. Altogether, our data show that the loss of GW2.1 gene activity in barley is associated with pleiotropic effects negatively affecting the development of generative organs and consequently the grain production. Our findings contribute to the better understanding of grain development and the utilisation of GW2.1 control in quantitative and qualitative genetic improvement of barley.

Optimum total nitrogen application is required to reduce the yield loss of hybrid rice to high temperature

The grain yield and quality of rice (Oryza sativa L.) face great challenges associated with increasing temperatures, thus threatening global food security. The wheat, maize and barley yield loss magnitudes imposed by increasing temperatures reportedly interact with the nitrogen (N) application level under field conditions. However, little is known about the interactive effect between high daytime temperatures and the N supply on the rice grain yield or quality or the influence of this effect on nitrogen use efficiency in the field. In this work, two hybrid rice varieties (Liangyoupeijiu-LYPJ and IIyou602-IIY602) were grown under three N application levels (N75–75 kg ha−1, N150–150 kg ha−1, and N225–225 kg ha−1) in paddy fields in 2019 and 2020 under control and higher-temperature (HT) conditions from the heading stage to maturity. HT exposure significantly decreased the grain yields, grain filling, grain weight, grain-leaf ratio, total biomass at maturity and harvest index (HI) of the two rice varieties at all N levels across both years, while the spikelet m−2 was only affected in 2019. Moreover, the grain yield reduction caused by HT were least at N150 (57.0%) in LYPJ (68.7% and 68.8% in N75 and N225, respectively) and N75 (60.7%) in IIY602(66.0% and 64.5% in N150 and N225, respectively) on average of two years. Grain filling percentage, biomass and HI also had least decreases under N150 in LYPJ and under N75 in IIY602. Regarding the grain quality, HT exposure decreased the head rice yield and grain length, and increased chalkiness in the two varieties in both years while decreasing the amylose content and increasing the protein content in 2019 and 2020, respectively. Relatively optimal N application rates (N75 or N150 for both varieties) improved grain quality indicators such as the brown rice, head rice, grain length, chalkiness degree and amylose content under HT. After HT exposure, nonsignificant changes in N accumulation at maturity and significant reduction in N translocation and N use efficiencies for grain and biomass production were observed in both varieties. Compared to the relatively higher N applications, applying N150 to LYPJ (by 24.3%) and N75 to IIY602 (by 33.7%) induced lower N translocation reductions. Thus, our results suggest that increasing N supply cannot minimize the damage caused by HT in the realistic fields.

High-Resolution Mapping of Barley mild mosaic virus Resistance Gene rym15.

Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), which are transmitted by the soil-borne plasmodiophorid Polymyxa graminis, cause high yield losses in barley. In previous studies, the recessive BaMMV resistance gene rym15, derived from the Japanese landrace Chikurin Ibaraki 1, was mapped on chromosome 6HS of Hordeum vulgare. In this study, 423 F4 segmental recombinant inbred lines (RILs) were developed from crosses of Chikurin Ibaraki 1 with two BaMMV-susceptible cultivars, Igri (139 RILs) and Uschi (284 RILs). A set of 32 competitive allele-specific PCR (KASP) assays, designed using single nucleotide polymorphisms (SNPs) from the barley 50 K Illumina Infinium iSelect SNP chip, genotyping by sequencing (GBS) and whole-genome sequencing (WGS), was used as a backbone for construction of two high-resolution maps. Using this approach, the target locus was narrowed down to 0.161 cM and 0.036 cM in the Igri × Chikurin Ibaraki 1 (I × C) and Chikurin Ibaraki 1 × Uschi (C × U) populations, respectively. Corresponding physical intervals of 11.3 Mbp and 0.281 Mbp were calculated for I × C and C × U, respectively, according to the Morex v3 genome sequence. In the 0.281 Mbp target region, six high confidence (HC) and two low confidence (LC) genes were identified. Genome assemblies of BaMMV-susceptible cultivars Igri and Golden Promise from the barley pan-genome, and a HiFi assembly of Chikurin Ibaraki 1 together with re-sequencing data for the six HC and two LC genes in susceptible parental cultivar Uschi revealed functional SNPs between resistant and susceptible genotypes only in two of the HC genes. These SNPs are the most promising candidates for the development of functional markers and the two genes represent promising candidates for functional analysis.

Genome-Wide Association Study for Powdery Mildew and Rusts Adult Plant Resistance in European Spring Barley from Polish Gene Bank

Rusts and powdery mildew are diseases that have a major effect on yield loss in barley. Adult Plant Resistance (APR) is a post-seedling resistance mechanism and its expression is influenced by many factors, including host susceptibility and weather conditions, as well as the timing and severity of disease outbreaks. There are two mechanisms associated with APR: non-hypersensitive and minor gene APR. In this study, 431 European barley accessions were evaluated phenotypically over 2 years (2018–2019) under field conditions, scoring APR to powdery mildew (PM), barley brown rust (BBR), and stem rust (SR), and genotypically using DArTseq. Accessions were grouped into sub-collections by cultivation period (group A—cultivated prior 1985, B—cultivated after 1985, and C—Polish landraces) and by European country of origin or European region. GWAS was conducted for PM, BBR, and SR, and scored at the heading (HA) and milky-waxy (MW) seed stages in 2019 and maximum scores across all replicates were obtained 2018–2019. Disease severity was sufficient to differentiate the collection according to cultivation time and country of origin and to determine SNPs. Overall, the GWAS analysis identified 73 marker–trait associations (MTAs) with these traits. For PM resistance, we identified five MTAs at both the HA stage and when considering the maximal disease score across both growth stages and both years. One marker (3432490-28-T/C) was shared between these two traits; it is located on chromosome 4H. For BBR resistance, six MTAs at HA and one MTA at the MW stage in 2019 and seven MTAs, when considering the maximal disease score across both growth stages and both years, were identified. Of the 48 markers identified as being associated with SR resistance, 12 were on chromosome 7H, 1 was in the telomeric region of the short arm, and 7 were in the telomeric region of the long arm. Rpg1 has previously been mapped to 7HS. The results of this study will be used to create a Polish Gene Bank platform for precise breeding programs. The resistant genotypes and MTA markers will serve as a valuable resource for breeding for PM, BBR, and SR resistance in barley.

Assessment of crop damage by rodent pests from experimental barley crop fields in Farta District, South Gondar, Ethiopia.

This study was conducted in Farta district, south Gondar from 2019 to 2020 cropping years to identify rodent pest species and estimate damage caused on barley crops. Four independent barley crop fields (40 x 40 m each) were sampled randomly to estimate the loss. Two were located near Alemsaga Priority State Forest and the other two were away from the forest. Four (2 x 2 m) rodent exclusion plots were established at 10 m interval as control units in each selected experimental barley fields using fine wire mesh. Rodent pest species were collected using both Sherman and snap traps throughout the different crop growing stages. The damaged and undamaged barley tillers by pest rodents were counted on five 1 x 1 m randomly sampled quadrats for each selected experimental fields. Variations on pest rodent population between cropping years and sites were analyzed using Chi square test. The mean crop damages between cropping years and experimental field sites were analyzed using two way ANOVA. Arvicanthis abyssinicus, Mastomys natalensis, Arvicanthis dembeensis, Mus musculus, Lophuromys simensis, Tachyoryctes splendens and Hystrix cristata were identified as pest rodents in the study area. A total of 968 individual rodents (427 in 2019 and 541 in 2020) were trapped during the study period. There was a statistical variation (χ2 = 13.42, df = 1 and P<0.05) between trapped individuals of the two successive years. The crop fields near the forest were more vulnerable than away from the forest during both cropping years. Statistical variations was observed on mean crop losses between cropping years and experimental barley crop sites. The highest crop damage was seen at maturity stage and the lowest during sowing in all experimental plots and cropping years. The percentage of barley yield loss due to rodent pests was 21.7 kg ha-1. The monetary value of this yield loss was equivalent to 4875 Birr (121.9 US$ h-1). Alemsaga Forest as shelter and conservation strategies like free of farmland from livestock and terracing for soil conservation have great role for the high rodent pest populations in the study area. Field sanitation, trapping and using restricted rodenticides like zinc phosphide are the possible recommendation to local farmers against rodent pests.

Rpg7: A New Gene for Stem Rust Resistance from Hordeum vulgare ssp. spontaneum.

Wheat stem rust (causal organism: Puccinia graminis f. sp. tritici) is an important fungal disease that causes significant yield losses in barley. The deployment of resistant cultivars is the most effective means of controlling this disease. Stem rust evaluations of a diverse collection of wild barley (Hordeum vulgare ssp. spontaneum) identified two Jordanian accessions (WBDC094 and WBDC238) with resistance to a virulent pathotype (P. graminis f. sp. tritici HKHJC) from the United States. To elucidate the genetics of stem rust resistance, both accessions were crossed to the susceptible landrace Hiproly. Segregation ratios of F2 and F3 progeny indicated that a single dominant gene confers resistance to P. graminis f. sp. tritici HKHJC. Molecular mapping of the resistance locus was performed in the Hiproly/WBDC238 F2 population based on 3,329 single-nucleotide polymorphism markers generated by genotyping-by-sequencing. Quantitative trait locus analysis positioned the resistance gene to the long arm of chromosome 3H between the physical/genetic positions of 683.8 Mbp/172.9 cM and 693.7 Mbp/176.0 cM. Because this resistance gene is novel, it was assigned the new gene locus symbol of Rpg7 with a corresponding allele symbol of Rpg7.i. At the seedling stage, Rpg7 confers resistance against a number of other important P. graminis f. sp. tritici pathotypes from the United States (MCCFC, QCCJB, and TTTTF) and Africa (TTKSK) as well as an isolate (92-MN-90) of the rye stem rust pathogen (P. graminis f. sp. secalis) from Minnesota. The resistance conferred by Rpg7 can be readily transferred into breeding programs because of its simple inheritance and clear phenotypic expression.

Drought assessment on barley and millet production in Karnali Province, Nepal

Spatio-temporal characteristics of agricultural drought and its impact on the yield of winter barley and summer millet crops in Karnali Province of Nepal were evaluated. For this purpose, precipitation data from 1988 to 2018 of 18 stations and agricultural data from 2003 to 2018 were used for the analysis. Standardized Precipitation Indices (SPI-3 and SPI-4) were used for measuring drought intensity. Based on drought intensity, SPI&lt;-1 was considered for drought occurrence. Similarly, Standardized Residual Yield Series (SYRS) was also calculated for crop yield change, eliminating other factors responsible for crop yield. The result shows that Salyan, Surkhet, and Jumla districts have more occurrence of summer and winter drought in comparison to other districts. Similarly, 1999, 2002, 2004-05, 2008-09, 2012, and 2015 were considered as drought years in most stations. Moreover, the province experienced more winter drought events than summer and winter droughts are increasing after 2015. Concerning the impact of drought occurrence on cereal yield loss, barley seems to be more affected. SPI-3 and SYRS (barley) are significantly correlated in Jumla, Humla, Mugu, Jajarkot, Dailekh, Surkhet, and Salyan districts at 95% confidence. With the exception, drought occurrence has a larger impact on barley yield loss than drought severity in the study area. However, millet yield loss doesn't seem to be affected due to drought occurrence and intensity in all districts except Rukum, as millet is a drought-tolerant crop. Therefore, for sustainable agriculture practices, drought-tolerant crops like millet could be suitable in Karnali Province, where drought frequency is very high.

High-resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease

Key messageWe mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The co-segregating markers will be instrumental for marker-assisted selection in barley breeding.Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.

Races of Puccinia graminis on barley, oat, and wheat in Canada in 2013 and 2014

Stem rust, caused by Puccinia graminis, is a catastrophic disease that has and continues to cause yield losses in barley, oat, and wheat crops worldwide. Use of host resistance genes is the most economic, efficient, and environmentally best method to control the disease. Resistance genes have been incorporated into most commercial cultivars and have been effective, for races in the North American populations of P. graminis. However, new races of stem rust are constantly emerging and therefore it is imperative to monitor the virulence structure in the populations to detect new races with increased virulence. Stem rust samples were collected in 2013 and 2014 in Manitoba, Ontario, Quebec, and Saskatchewan to determine the incidence and severity of stem rust in barley (Hordeum vulgare), oat (Avena sativa), and wheat (Triticum aestivum) fields and to characterize the virulence dynamics in the pathogen populations. No stem rust was found in cultivated wheat, and incidence was at trace (<1%) levels in barley fields and very low (0–5%) levels in oat fields in 2013 and 2014. In wheat trap plots and collections from barley, race QFCSC of P. graminis f. sp. tritici was dominant in 2013 (85.9%) and was the only race found in 2014. Races MCCFC, QTHJF, RFCSC, RKQSC, and TMRTF were at low (<5%) levels in 2013. From collections in oat fields and stands of wild barley, nine races of P. graminis f. sp. avenae were found in 2013, with TJS (56.9%) and TJJ (23.5%) the most frequent. In 2014, we found only six races and TJS (74.5%) and TJJ (19.1%) were the most frequent. No new races of stem rust were detected in Canada in 2013 or 2014.

Antagonistic potential of some bacterial strains against Xanthomonas campestris, the cause of bacterial blight in Hordeum vulgare

Bacterial blight disease due to Xanthomonas campestris pv. translucens results in yield losses in barley, Hordeum vulgare L., especially in warm climates. Bio-based bactericides represent a safe alternative to harmful chemicals for controlling a wide range of phytopathogens. The bacterial strains (Brevibacterium linens, Bacillus subtilis, B. thuringiensis) were tested as antagonistic potential against X. campestris disease in barley seedlings. Antagonists were applied as seed biopriming and soil drench in X. campestris infested soil. Soil-drenching treatment was more efficient than the biopriming application. A significant increase in shoot length with a clear decrease in seed germination was recorded. Fresh and dry weights of shoot and root lengths of the treated plants were markedly improved. The remarkable antagonistic activity of B. linens, B. subtilis, and B. thuringiensis against X. campestris could be attributed to the capability to produce bioactive molecules that can trigger systemic resistance in the infected seedlings.

The genotypic difference in the effect of water stress after anthesis on the malt quality parameters in barley

It is well known that water stress is a major abiotic factor causing barley yield loss and unstable malt quality. However it is not quite clear about the altered pattern of the main malt quality traits for the barley genotypes differing in water stress tolerance. In this study, the effect of water stress during grain filling stage on grain weight, β-glucan content, β-amylase and limit dextrinase (LD) activities of three Tibetan wild and two cultivated barley genotypes were investigated. The results showed that water stress reduced biomass accumulation and grain weight of all genotypes, and increased protein content, but the reduced or increased extent differed significantly among genotypes, with XZ147 being less affected. In general, water stress increased β-amylase activity, reduced β-glucan content, had little effect on LD activity, which are attributed to increased protein and hordein contents, reduced grain weight, and its ineffective form in grains, respectively. Tibetan wild barley XZ147 is characterized by little change in grain weight and β-glucan content, dramatically increase of β-amylase activity under water stress, indicating its great potential for use in developing barley cultivars with high tolerance to water stress and high malt quality.

Identification of genomic regions involved in tolerance to drought stress and drought stress induced leaf senescence in juvenile barley.

BackgroundPremature leaf senescence induced by external stress conditions, e.g. drought stress, is a main factor for yield losses in barley. Research in drought stress tolerance has become more important as due to climate change the number of drought periods will increase and tolerance to drought stress has become a goal of high interest in barley breeding. Therefore, the aim is to identify quantitative trait loci (QTL) involved in drought stress induced leaf senescence and drought stress tolerance in early developmental stages of barley (Hordeum vulgare L.) by applying genome wide association studies (GWAS) on a set of 156 winter barley genotypes.ResultsAfter a four weeks stress period (BBCH 33) leaf colour as an indicator of leaf senescence, electron transport rate at photosystem II, content of free proline, content of soluble sugars, osmolality and the aboveground biomass indicative for drought stress response were determined in the control and stress variant in greenhouse pot experiments. Significant phenotypic variation was observed for all traits analysed. Heritabilities ranged between 0.27 for osmolality and 0.61 for leaf colour in stress treatment and significant effects of genotype, treatment and genotype x treatment were estimated for most traits analysed. Based on these phenotypic data and 3,212 polymorphic single nucleotide polymorphisms (SNP) with a minor allele frequency >5 % derived from the Illumina 9 k iSelect SNP Chip, 181 QTL were detected for all traits analysed. Major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. BlastX search for associated marker sequences revealed that respective SNPs are in some cases located in proteins related to drought stress or leaf senescence, e.g. nucleotide pyrophosphatase (AVP1) or serine/ threonin protein kinase (SAPK9).ConclusionsGWAS resulted in the identification of many QTLs involved in drought stress and leaf senescence of which two major QTLs for drought stress and leaf senescence were located on chromosome 5H and 2H. Results may be the basis to incorporate breeding for tolerance to drought stress or leaf senescence in barley breeding via marker based selection procedures.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0524-3) contains supplementary material, which is available to authorized users.

Quantitative trait loci controlling barley powdery mildew and scald resistances in two different barley doubled haploid populations

Powdery mildew and scald can cause significant yield loss in barley. In order to identify new resistance genes for powdery mildew and scald in barley, two barley doubled haploid (DH) populations were screened for adult plant resistance in the field and glasshouse under natural infection. The mapping populations included 92 DH lines from the cross of TX9425 × Franklin and 177 DH lines from the cross of Yerong × Franklin. Two quantitative trait loci (QTL) for resistance to powdery mildew were identified in the TX9425 × Franklin population. These QTL were mapped to chromosomes 7H and 5H, respectively. The phenotypic variation explained by the two QTL detected in this population was 22 and 17%, respectively. Three significant QTL were identified from the Yerong × Franklin population for the resistance to powdery mildew; the major one, detected on the short arm of chromosome 1H, explained 66% of phenotypic variation. The major QTL for scald resistance, identified from two different populations which shared a common parent, Franklin, were mapped in the similar position on 3H. However, the Franklin allele provided resistance to one population but susceptibility to the other population. The Yerong allele on 3H showed much better resistance to scald than the Franklin allele, which has not been reported before. Using high-density maps for both populations, some markers which were very close to the resistance genes were identified. Transgression beyond the parents in disease resistances of the DH populations indicates that both small-effect QTLs and genetic background may also have significant contributions towards the resistance.

Barley (Hordeum vulgare) and Wild Oat (Avena fatua) Competition Is Affected by Crop and Weed Density1

Wild oat is the most serious grass weed in Argentine barley crops and its control has concentrated on herbicide strategies. Increasing crop density could be an effective strategy to reduce the effect of wild oat on barley yield. However, limited research has been conducted to evaluate the effect of crop density on the competitive balance between barley and spontaneous populations of wild oat. A field experiment was conducted in 1992, 1993, and 1999, to study the effect of spontaneous populations of wild oat on barley sown at densities of 160, 220, and 280 plants/m2. Wild oat density averaged 84 plants/m2. Wild oat biomass increased linearly with weed density in all treatments but was reduced by increasing barley seeding rates. Barley biomass and yield were not affected by wild oat at high crop sowing densities, but for the low and medium barley densities, yield loss was almost 25% when 70 wild oat plants/m2 were established. Barley yield loss was mostly related to competition from the early emerged wild oat. The relationship between yield losses and wild oat density was equally significant when the whole population or only early emerged individuals of the weed were considered.Nomenclature: Wild oat, Avena fatua L. #3 AVEFA; barley, Hordeum vulgare L.Additional index words: Wild oat interference in barley, integrated wild oat management, seeding rates of barley.

Rym15 from the Japanese cultivar Chikurin Ibaraki 1 is a new barley mild mosaic virus (BaMMV) resistance gene mapped on chromosome 6H.

Breeding for resistant cultivars is the only way to prevent high yield loss in barley caused by the soil-borne barley mild mosaic virus (BaMMV) complex. We have characterized the BaMMV resistance of barley cv. Chikurin Ibaraki 1. Doubled haploid lines were obtained from the F(1) between the susceptible six-rowed winter barley cultivar, Plaisant, and Chikurin Ibaraki 1. Each line was tested for reaction to BaMMV by mechanical inoculation followed by DAS-ELISA. Of 44 microsatellites that covered the genome, 22 polymorphic markers were tested on one susceptible and one resistant bulk, each comprising 30 lines. Differential markers and additional microsatellite markers in the same region were then tested on the whole population. A bootstrap analysis was used to compute confidence intervals of distances and to test the orders of the resistance gene and the closest markers. A segregation of 84 resistant/98 susceptible lines fitted a 1:1 ratio (chi(2)=1.08, P=0.30), which corresponds to a single gene in this DH lines population. The resistance gene was flanked by two markers near the centromeric region of chromosome 6HS-Bmag0173, at 0.6+/-1.2 cM, and EBmac0874, at 5.8 +/- 3.4 cM. We propose to name this new resistance gene rym15. This resistance gene and associated markers will increase the possibilities to breed efficiently for new cultivars resistant to the barley mosaic disease.

Wild Oat (Avena fatua) Interference in Barley (Hordeum vulgare) is Influenced by Barley Variety and Seeding Rate1

Abstract: Field experiments were conducted at Vegreville and Lacombe, AB, to determine the influence of barley (Hordeum vulgare) variety and seeding rate on interference of wild oat (Avena fatua) with barley. Barley variety and seeding rate affected barley density, height at maturity, and seed yield, as well as wild oat shoot dry weight and seed yield in most experiments, but there was no variety by seeding rate interaction. As expected, the semidwarf varieties Falcon and CDC Earl were the shortest. Barley seedling emergence and subsequent plant densities varied among varieties, locations, and years. The hull-less varieties Falcon and CDC Dawn had the poorest emergence in most cases, whereas AC Lacombe and Seebe had the highest emergence. Wild oat shoot dry matter and seed production was highest in the Falcon, CDC Dawn, and CDC Earl plots, suggesting that these were the least competitive with wild oat. Barley yield loss from wild oat interference also tended to be highest in these varieties. Poor emergenc...

Effect of barley plant density on wild oat interference, shoot biomass and seed yield under zero tillage

There has been little research aimed at developing regression models to describe the effects of barley and wild oat plant density on barley yield loss, or wild oat biomass and seed yield. Such models are an important component of integrated weed management systems, and can help determine when weed control with herbicides is economical. Field experiments were conducted over 4 yr at Vegreville, Alberta, to determine the interactive effects of wild oat and barley plant density on barley and wild oat variables in a zero tillage system. A nonlinear regression model in most cases provided good descriptions of barley yield loss, wild oat shoot dry weight, and wild oat seed yield as functions of wild oat and barley plant densities. The interactive effect of wild oat and barley plant density on percentage barley yield loss did not differ significantly (P = 0.05) among years. A pooled regression model describing barley yield loss accounted for 57% of the variation, and provides a means of estimating yield loss due to wild oat in barley grown under zero tillage. Barley yield loss increased as wild oat density increased but the magnitude of the yield loss diminished with increasing barley plant density. Wild oat economic threshold densities varied among years, and were strongly influenced by barley price and expected wild oat-free yield. Economic thresholds were greater at higher barley plant densities. Barley seed weight decreased with increasing barley plant density, and to a lesser extent with increasing wild oat density. The interactive effect of wild oat and barley plant density on wild oat seed yield varied significantly with year, and appeared to be influenced by climatic conditions. The cooler, wetter spring of 1996 favored wild oat seed production (by several orders of magnitude) compared with the relatively warmer and drier spring of 1995. Each year wild oat seed yield and shoot dry weight decreased as barley plant density increased. The results suggest that seeding barley at relatively high rates may reduce the need for wild oat control with herbicides in zero tillage systems. Key words: Zero tillage, wild oat interference, barley seeding rate, nonlinear regression

Races ofPuccinia graminisin the United States During 1990

Wheat stem rust overwintered in southern and east central Texas, southern Louisiana, and southeastern Alabama. Yield losses to stem rust of wheat were traces, but a 1% yield loss in barley occurred in North Dakota. Race Pgt-QCCJ was the most common race, making up 50% of the 450 isolates from 170 collections. If only the 89 collections from wheat were considered, the most common races were Pgt-TPMK, QFCS, and QCCJ, comprising 53, 21, and 16% of the isolates, respectively. Eighty percent of the isolates of QCCJ were from cultivated barley, and 90% of all isolates from cultivated barley were race QCCJ. No virulence was found to wheat lines with «single» genes Sr13, 22, 24, 25, 26, 27, 29, 30, 31, 32, 33, 37, Gt, or Wld-1

Disease progression and yield loss in barley associated with net blotch, as influenced by fungicide seed treatment

Fungicide seed treatment significantly reduced the severity of net blotch of barley induced by Pyrenophora teres. Triadimenol and maneb treatments were more effective than etaconazole, Vitaflo 280 (carbathiin plus thiram), or Vitaflo 250 (carbathiin). Disease severity at growth stage 73 (Zadoks) was reduced on the penultimate leaf from maneb and triadimenol seed treatments. Apparent infection rate (r) did not effectively identify relative treatment efficacy or potential. Estimated time to 50% disease severity on the second to fourth leaf from the head, based on logit disease transformation, was delayed by 2.5 to 9.2 days with triadimenol treatment. Triadimenol and maneb treatments provided a 12.6% and 12.4% yield increase, respectively, which was in part directly related to control of net blotch. Significant correlations of yield and thousand kernel weight to percent net blotch on the penultimate, third, and fourth leaves were obtained. It was estimated that net blotch on Volla barley was responsible for a 15.2% yield loss and 6.7% reduction in thousand kernel weight. Estimations of relative treatment efficacy and of yield loss were more reliable when based on disease assessments of the fourth leaf from the head than from higher leaf positions.

AN APPROACH TO ESTIMATING YIELD LOSS OF BARLEY DUE TO CANADA THISTLE

Experiments were conducted to determine if a relationship exists between yield loss of barley and various indicators of Canada thistle density. Correlation coefficients between barley yields and total numbers of Canada thistle shoots/m2, numbers of shoots/m2 taller than 0.5 m, numbers of flowering shoots/m2 or shoot dry weight (g/m2), using two representations of the density data (untransformed or square root transformation) were significant at P &lt; 0.01. The data for percent yield loss of barley and total numbers of Canada thistle shoots/m2 were analyzed by regression using both representations of the data and yielded the following equations:[Formula: see text]and[Formula: see text]where ŷ = estimated percent yield loss of barley, x = the total number of Canada thistle shoots/m2 and 0.85 and 7.6 were the indices of competition for Eqs. 1 and 2, respectively. Equation 2 probably provides a more accurate estimate of barley yield loss at low levels of Canada thistle infestation [Formula: see text] whereas either equation is acceptable at intermediate-to-high levels of infestation (5–45 shoots/m2). The 95% confidence limits for Eqs. 1 and 2 and for the estimated percent barley yield losses when four different sample sizes (n = 1, 5, 10 or 20) are used to estimate the thistle density mean are presented. The Eq. 2 index of competition is extrapolated to other crops. Comparing a Canada thistle shoot with a wild oat plant in barley, Canada thistle may be about 3.4 times as competitive as wild oats.