Abstract
Key messageWe mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The co-segregating markers will be instrumental for marker-assisted selection in barley breeding.Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.
Highlights
Viruses are an increasing threat to crops worldwide
It is known that the penetrance of infection in such experiments is never complete, and that the genetic background of the plant plays a role in this phenomenon, Maris Otter being the most susceptible cultivar tested (Adams et al 1986, 1993)
Among the HC genes, HORVU.MOREX. r2.6HG0448010 is annotated as a TIR-NBS-LRR gene in Monat et al (2019), our analysis reveals that it does not contain any of the major domains of nucleotidebinding and leucine-rich repeat domain (NLR) genes (TIR or coiled-coil, NB-ARC and LRR), and monocotyledons so far have not been shown to contain TIR-NLR genes (Jacob et al 2013)
Summary
Viruses are an increasing threat to crops worldwide. The soil-borne barley yellow mosaic disease, caused by a complex of two Bymoviruses (Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV)) is one Communicated by Kevin Smith. Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. KWS SAAT SE & Co. KGaA, Grimsehlstr. As chemical control of those viruses, transmitted by the plasmodiophorid Polymyxa graminis (Kanyuka et al 2003), is not possible, only the use of resistant varieties can preserve yield in infected fields
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