Goal. To establish the expediency and effect of using the nitrification inhibitor 3,4-dimethyl pyrazole phosphate (DMPF) in combination with urea-ammonia mixture (KAS-32) on the yield of winter wheat grain. To determine the difference in the yield of winter wheat using different norms of KAS-32 with and without the addition of DMPF. Methods. Field (stationary experiment); laboratory (agrochemical analysis of soil and plant samples); analytical (systematic analysis of long-term results of field experiments and meteorological observations using mathematical and statistical methods, correlation and regression analysis, mathematical modeling, and empirical generalization of established patterns). Results. It was confirmed that the use of DMPF together with KAS-32 significantly influenced the yield of winter wheat. At the same rate of KAS-32, 300 kg/ha in the variant with the use of DMPF, the yield increased by 4.5 t/ha, or 12% compared to the option where KAS-32 was used at the same rate, 300 kg/ha without the use of DMPF. In the variant with a reduced rate of KAS-32, 250 kg/ha with the use of DMPF compared to the option with an increased rate of KAS-32, 300 kg/ha, but without the use of DMPF, the yield increased by 4.2 c/ha or 11.2% over 4 years of research (2018–2021). Conclusions. It was established that in the variants using DMPF together with KAS-32, the productivity increased compared to the control (without fertilizers) by 25.3–25.6 t/ha (67.6–68.4%). In the variants using DMPF together with KAS-32 compared to the variant using KAS-32, but without the use of DMPF, the yield increased by 4.2–4.5 t/ha (11.2–12.0%) over 4 years of research (2018–2021)).