Wound Healing Activity Research Articles

Antibacterial and Wound Healing Assessment of Silver Nanoparticles against Multidrug-Resistant Klebsiella variicola

The increase in antimicrobial resistance and the absence of novel antibiotic development cause problems in controlling infections for wound healing. Antibacterial properties of nanomaterials have emerged as a potentially effective approach in the pursuit of superior alternatives, nevertheless, the toxicity associated with higher concentrations has emerged as a significant obstacle. According to this study, green nanoparticle synthesis is demonstrated to be both economical and biocompatible on account of the bioactive compounds present. In the present work, silver nanoparticles prepared by using Pistacia khinjuk gum, then, nanoparticle-based cream was prepared and compared with Fusidin and Vaseline creams via assessment in vitro antibacterial and in vivo wound healing activity on Wistar albino rats infected with multi drug resistant Gram negative bacteria which was newly recorded in Iraq, namely Klebsiella variicola. The findings revealed that the application of nanoparticle cream resulted in more rapid and effective wound healing (11 days), demonstrating a significant synergistic effect in comparison to Fusidin (16 days), Vaseline (20 days), and the untreated control group rats (32 days). The results indicated that green nanoparticles proved to be a significant strategy in the fight against multidrug resistant bacteria, without any toxicity concerns.

Enhanced wound healing activity of naturally derived Lagenaria siceraria seed oil binary nanoethosomal gel: formulation, characterization, in vitro/in vivo efficiency

BackgroundThe present study aims to enhance the wound healing potential of the seed oil (SO) of Lagenaria siceraria (Egyptian cultivar) via the preparation of SO-loaded binary nanoethosomal (SO-BNE) gels. SO-BNEs were prepared using 23 factorial design, characterized for vesicle size, zeta potential, polydispersity index, linoleic and oleic acid EE% for ensuring improved skin permeability. The L. siceraria SO, optimized SO-BNE gels (0.5% and 5%) and Mebo® were topically applied in full-thickness wounded rat model twice daily for 10 days.ResultsIn the SO-BNE gel groups, the normal appearance of the skin architecture and structure of the dermis was revealed. In addition, the levels of NRF2, TGF-β1 and FOXO1, collagen type I, SMA-α and MIP2 were significantly elevated. The wound healing potential of SO-BNE gels was proposed to be via suppression of oxidative stress and stimulation of skin regeneration biomarkers. Furthermore, the SO screening through GC/MS unveiled high percentages of unsaturated fatty acids. SO was also found to be nontoxic to human skin fibroblast cells; enhanced viability and migration rates at concentration of 50 g/mL by 99.76% and 75.9%, respectively.ConclusionThese findings demonstrate that the Lagenaria siceraria SO-loaded BNE gels represent a promising delivery for wound healing with enhanced release and bioavailability.

Burn Wound Healing Activity of Hydroxyethylcellulose Gels with Different Water Extracts Obtained from Various Medicinal Plants in Pseudomonas aeruginosa-Infected Rabbits.

Burn injuries represent a significant problem in clinical practice due to the high risk of infection and the prolonged healing process. Recently, more attention has been given to natural remedies such as water extracts of various medicinal plants, which possess anti-inflammatory and wound healing properties. The aim of this study is to evaluate the efficacy and safety of Satureja montana L. and other water extracts in a burn wound model. The study involved male Californian rabbits (n = 52) divided into eight groups. Burn wounds were modeled on the animals and subsequently treated with gels based on Satureja montana L. and other water extracts. The reparative potential of the epidermis (assessed by Ki-67 expression), the state of local immunity (measured by the number of CD-45 cells), and the anti-inflammatory role of mast cells (measured by tryptase levels) were evaluated. Bacteriological and morphological studies were conducted. The most pronounced bactericidal, reparative, and immunostimulatory effects were observed after the treatment using a gel mixture of water extracts from Satureja montana L., Salvia sclarea, Coriandrum sativum L., and Lavandula angustifolia in equal proportions (1:1:1:1). The other gels also demonstrated high efficacy in treating burn wounds, especially when using a strain of Pseudomonas aeruginosa resistant to several antibiotics. Immunohistochemical studies showed a significant increase in the number of Ki-67-positive cells in the basal layer of the epidermis and a decrease in the number of CD-45-positive cells, indicating improved proliferative activity and reduced inflammation. This study confirms the hypothesis that the use of water extract mixtures significantly enhances the reparative potential, improves the immune response in the treatment of burns, and promotes wound healing. These findings pave the way for further research and the application of complex phytotherapeutic agents, specifically water extracts of medicinal plants containing phenols and antioxidants in burn wound therapy.

Pyroxylin-based liquid bandage forms a mechanically active protective film to facilitate skin wound healing in mice

Wound healing requires interplay between cells and molecules. Recent evidence has demonstrated that liquid bandages promote wound healing by forming a protective barrier against contamination, attenuating inflammation, and enhancing re-epithelialization and angiogenesis. In this study, we evaluated the wound healing activity of pyroxylin-based liquid bandage (LiQuiPlast®) in eight-week-old C57BL/6 male mice by generating a single 4 mm diameter full-thickness excisional skin wound on the dorsum. In the LiQuiPlast® group, the liquid bandage was applied on day 0 and was replaced every four days. Wound size was monitored every day for two weeks. The results showed that LiQuiPlast® was mechanically active (induced wound contraction), which promoted a significant wound size reduction (27 %−39 %, compared to the control group) on days 1–4 postinjury. In addition, a significant reduction in wound size was observed again in the LiQuiPlast® group (25 %−29 %, compared to the controls) on days 8−9 postinjury. LiQuiPlast®-treated wounds showed no scab. Immunohistochemistry analyses displayed a reduction in neutrophils and tumor necrosis factor-α levels in LiQuiPlast®-treated wounds, compared to the control group on day 4 postinjury (the inflammatory phase). In addition, LiQuiPlast®-treated mice had enhanced keratinocyte proliferation than control mice during this time. On day 13 postinjury, LiQuiPlast® significantly reduced hypertrophic scarring and enhanced expression and reorganization of collagen fiber compared to control mice. In conclusion, we show that LiQuiPlast® acts as a mechanically active protective film, which promotes moist wound healing by promoting wound contraction, no scab formation, attenuated inflammation, enhanced keratinocyte proliferation, and decreased scarring.

Design and Development of a Self-nanoemulsifying Drug Delivery System for Co-delivery of Curcumin and Naringin for Improved Wound Healing Activity in an Animal Model.

The present study endeavored to design and develop a self-nanoemulsifying drug delivery system to improve the solubility and dermatological absorption of curcumin and naringin. Curcumin and naringin-loaded self-nanoemulsifying drug delivery system formulations were developed using aqueous phase titration. Phase diagrams were used to pinpoint the self-nanoemulsifying drug delivery system zones. Tween 80 and Labrasol (surfactants), Transcutol (cosurfactant), and cinnamon oil were chosen from a large pool of surfactants, cosurfactants, and oils based on their solubility and greatest nano-emulsion region. Fourier transform infrared spectroscopy, zeta sizer, and atomic force microscopy were used to characterize the optimized formulations and test for dilution and thermodynamic stability. The optimized curcumin-naringin-self-nanoemulsifying drug delivery system demonstrated the following characteristics: polydispersity index (0.412 ± 0.03), % transmittance (97%), particle size (212.5 ± 05 nm), zeta potential (- 25.7 ± 1.80 mV) and having a smooth and spherical droplet shape, as shown by atomic force microscopy. The ability of their combined formulation to cure wounds was tested in comparison to pure curcumin suspension, empty self-nanoemulsifying drug delivery system, and standard fusidic acid. Upon topical administration, the optimized curcumin-naringin-self-nanoemulsifying drug delivery system demonstrated significant wound healing activity in comparison with a pure curcumin suspension, empty self-nanoemulsifying drug delivery system, and standard fusidic acid. Based upon this result, we assume that skin penetration was increased by using the optimized curcumin-naringin-self-nanoemulsifying drug delivery system with enhanced solubility.

BURN WOUND HEALING ACTIVITY OF ETHANOL EXTRACT OF ECENG GONDOK (Eichornia crassipes) LEAVES

Eceng gondok is a weed plant that lives in shallow water areas. The aim of this research was to determine the burn wound healing activity of an ethanol extract of Eceng gondok leaves. The study utilized 28 male mice with burn injuries as test animals, dividing them into 4 groups. We gave a gel containing an ethanol extract to the 10% group. The 0% group received a gel base; the positive control group received Bioplacenton® gel; and the negative control group received no treatment. For 14 days, we administered treatment to all treatment and control groups once a day and measured the burn wound's diameter using the Morton method every two days. Data shown were significant differences between the 10% extract group and the positive control groups. The concentration that is effective in burn wound healing is 10%, with the smallest wound diameter and the largest healing percentage.

Green synthesis of zinc oxide nanoparticles from Wodyetia bifurcata fruit peel extract: multifaceted potential in wound healing, antimicrobial, antioxidant, and anticancer applications.

This study focuses on the synthesis, characterization, and use of zinc oxide nanoparticles (ZnONPs) derived from W. bifurcata fruit peel extract. ZnONPs are frequently synthesized utilizing a green technique that is both cost-effective and ecologically friendly. ZnONPs were characterized utilizing analytical techniques. Ultra Violet visible (UV-Vis) spectra showed peaks at 364nm, confirming the production of ZnONPs. Scanning Electron Microscope analysis indicated that the nanoparticles generated were spherical/agglomerated, with diameters ranging from 11 to 25nm. FTIR spectroscopy was used to identify the particular functional groups responsible for the nanoparticles' reduction, stabilization, and capping. Phytochemical analysis of the extract revealed that flavonoids, saponins, steroids, triterpenoids, and resins were present. The antibacterial activity of W. bifurcata synthesised nanoparticles was evaluated against pathogenic bacteria. The ZnONPs antioxidant activity was assessed using DPPH assay. The in vitro cytotoxicity was assessed against prostate cancer PC3 cells. The wound healing potential was assessed by employing in vitro scratch assay and in vivo excision model in Wistar rats. Because of its environmentally benign production, low toxicity, and biocompatibility, ZnONPs exhibited potential antibacterial, antioxidant, anticancer, and wound healing activities, indicating that they could be used in cancer treatment and wound management. Further study is required to examine the fundamental mechanisms and evaluate the safety and effectiveness of the test sample in clinical situations.

Portulaca grandiflora phytochemicals as a potential source for wound healing activity: in vitro and in vivo studies

The management of chronic wounds presents considerable challenges and has a substantial influence on the quality of life among afflicted persons and their families. The discovery of naturally produced bioactive chemicals having good effects on the regeneration of tissue has been driven by the need for safe, effective, and cost-effective wound healing therapies. Portulaca grandiflora is an annual succulent that comes under the family of Portulacaceae. The purpose of this study was to evaluate the phytochemicals of n-hexane extracts and their effects on in vitro and in vivo wound healing models. In vitro study, Wound Scratch Assay shows that n-hexane extract of 100 ug/ml concentration significantly promotes wound healing through increased cell migration and wound closure compared to MEBO ointment-treated groups and negative control groups. The in vivo study shows that the formula of the extract ointment promotes wound healing without any complication and the percentage of wound surface area reduction was (5.39 ± 0.391) better than MEBO-treated groups (10.06 ± 0.536), and negative control groups (14.04 ± 0.304) through its ability to stimulate epidermal closure, re-epithelization, and granulation that appears in histological examination. As a result, Iraqi Portulaca grandiflora can be considered a potential resource of chemical substances, especially terpenoids, and steroids, which are helpful in healing wounds.

Therapeutic potential of cannabis for surgical wound healing in rats.

This study was conducted to evaluate the wound-healing activities of a Cannabis sativa L. plant extract and cannabidiol on incision wounds. An incision was created and sutured in rats under anaesthesia. Routine wound care procedures were applied for 10 days, followed by histological wound examinations. The cellular bioactivities of the hemp extract and CBD were assessed for MCP-1, EGF, BFGF, IL-8, and COL-1 using ELISA on the rat skin wound healing activity. A one-way ANOVA was used for the data analysis. The EGF values in the plasma were similar in the povidone-iodine, hemp seed oil, and hemp essential oil groups (P > 0.05). However, the EGF levels were lower in the CBD group compared to the other groups (P < 0.001, P < 0.005). The MCP-1 values in the hemp seed oil, hemp essential oil, and CBD were similar (P > 0.05), whereas povidone iodine exhibited lower MCP-1 levels compared to the other groups (P < 0.001, P < 0.005). It was determined that the plasma BFGF, IL-8, and COL 1 values of the groups were similar (P > 0.05). To our knowledge, this study is the first to evaluate the effects of CBD, seed oil, and hemp leaf extract on incision wound healing. It demonstrates that hemp extract holds greater potential benefits for wound healing compared to CBD.

The stress-responsive gene ATF3 drives fibroblast activation and collagen production through transcriptionally activating TGF-β receptor Ⅱ in skin wound healing.

Skin wound is an emerging health challenge on account of the high-frequency trauma, surgery and chronic refractory ulcer. Further study on the disease biology will help to develop new effective approaches for wound healing. Here, we identified a wound-stress responsive gene, activating transcription factor 3 (ATF3), and then investigated its biological action and mechanism in wound healing. In the full-thickness skin wound model, ATF3 was found to promote fibroblast activation and collagen production, resulted in accelerated wound healing. Mechanically, ATF3 transcriptionally activated TGF-β receptor Ⅱ via directly binding to its specific promoter motif, followed by the enhanced TGF-β/Smad pathway in fibroblasts. Moreover, the increased ATF3 upon skin injury was partly resulted from hypoxia stimulation with Hif-1α dependent manner. Altogether, this work gives novel insights into the biology and mechanism of stress-responsive gene ATF3 in wound healing, and provides a potential therapeutic target for treatment.

Wound Healing Activity of Phyllanthin-rich Sub-fractions Ointment: Isolated from Meniran (Phyllanthus niruri L.) Leaf in Experimental Rats Using Hydroxyproline as Biochemical Marker

Wound Healing Activity of Phyllanthin-rich Sub-fractions Ointment: Isolated from Meniran (Phyllanthus niruri L.) Leaf in Experimental Rats Using Hydroxyproline as Biochemical Marker

Antibacterial and Wound Healing Activity In Vitro of Individual and Combined Extracts of Tagetes nelsonii Greenm, Agave americana and Aloe vera

Currently, there are various physical and mechanical agents that can cause skin wounds, which are still traditionally treated with plant extracts. It has been reported that the genus Tagetes has a wide range of biological properties, including antibacterial and wound healing activity. Likewise, Agave americana extract and Aloe vera gel have shown potential in the treatment of burn wounds and other skin conditions both in vitro and in vivo. In this study, the antibacterial and wound healing activities of each of these plants were investigated, as well as the possibility of enhancing these activities by combining them. First, the secondary metabolites of the extracts were quantified, the antibacterial activity was evaluated using the Kirby-Bauer method, and their cytotoxicity was measured in 3T3 and HaCaT cells using the sulforhodamine B assay. The results revealed that Tagetes nelsonii extract had a higher amount of secondary metabolites, which is why it exhibited antibacterial activity. Finally, the scratch assay showed that the individual extracts of T. nelsonii and A. americana demonstrated greater cell migration and proliferation starting from 12 h, as well as when using the combination of A. americana extract and A. vera gel, which almost completely closed the wound compared to the control.

Potential Wound Healing and Anti-Melanogenic Activities in Skin Cells of Aralia elata (Miq.) Seem. Flower Essential Oil and Its Chemical Composition.

Aralia elata (Miq.) Seem. (AES; family Araliaceae) is a medicinal plant and has been reported to have various bioactivities, including anticancer and hepatotoxicity protective activities. However, no studies have investigated the biological activities of AES or its extracts on skin. To address this, we aimed to explore the effect of AES-flower-derived absolute-type essential oil (AESFEO) on skin-related biological activities, especially skin wound healing and whitening-related responses in skin cells (human-derived keratinocytes [HaCaT cells] and melanocytes [B16BL6 cells]) and to identify the components of AESFEO. Cell biological activities were analyzed using WST and BrdU incorporation assays, ELISA, or by immunoblotting. In HaCaT cells, AESFEO promoted proliferation, type IV collagen production, and enhanced the phosphorylations of Erk1/2, p38 MAPK, JNK, and Akt. In B16BL6 cells, AESFEO reduced serum-induced proliferation, α-MSH-stimulated increases in melanin synthesis and tyrosinase activity, and α-MSH-induced increases in MITF, tyrosinase, TRP-1, and TRP-2 expressions. In addition, AESFEO inhibited the phosphorylation of Erk1/2, p38 MAPK, and JNK in α-MSH-stimulated B16BL6 cells. Eighteen compounds were identified in AESFEO by GC/MS. These results suggest that AESFEO has beneficial effects on keratinocyte activities related to skin wound healing and melanocyte activities related to inhibition of skin pigmentation. AESFEO may serve as a useful natural substance for developing agents that facilitate skin wound healing and inhibit melanogenesis.

A Comparative Study of Two Oroxylum indicum (L.) Kurz. Phenotypes Based on Phytochemicals and Antioxidant Effects, and the Anti-Inflammatory Activity of Leaf and Pod Extracts.

Indian trumpet tree Oroxylum indicum (L.) Kurz exhibits a wide range of biological activities in all plant parts, including anti-inflammation, antioxidant, and wound-healing activities. In Thailand, there are tall- and short-stem phenotypes. The latter are preferred for commercial cultivation due to their fast growth and lower harvesting cost. This study aimed to compare the chemical profiles and antioxidant effects of leaves and young pods between two phenotypes using principal component analysis (PCA) and then to evaluate the anti-inflammatory potential of the selected phenotype's plant parts. The biomarker contents were quantified by HPLC. The antioxidants were determined using the DPPH, ABTS, and FRAP models. Nitric oxide (NO) production assays in LPS-induced RAW264.7 macrophages were performed to determine the anti-inflammatory property of the extracts. The PCA revealed that there were no differences in total phenolic content, total flavonoid content, or antioxidant activities between short- and tall-stem phenotypes. Higher potency of the NO-inhibitory effect was achieved from the leaf extract than the pod extract. These results support using the short-stem phenotypes for utilizing the leaf and pod of O. indicum, and suggest choosing the leaf part for further anti-inflammatory product development.

Updates on some medicinal and ornamental plants- Ayurvedic medicines

This literature review paper highlights morphology, distribution, medicinal properties of commercially important tree species, Thespesia populnea, Terminalia arjuna, Butea monosperma (Palas), Silver oak (Grevillea robusta), Mangifera indica, Madhuca longifolia,Melia azadirachta, Michelia champaca, Moringa oleifera, Peltophorum pterocarpum, and Aegle marmelos. These plants have been used for centuries by local communities and traditional healers for their therapeutic and medicinal properties. Medicinal plants play a crucial role in the Western Ghats, both ecologically and culturally. The use of herbal medicines for the treatment of diseases is safe and traditional. Therefore, medicinal plants have been receiving great attention because of their safety. The Western Ghats harbors more than 7,500 flowering plant species, of which nearly 1,500 are recognized for their medicinal values by local communities and traditional healers. The Western Ghats population traditional medical practices frequently center on using plant-based treatments. These medicinal plants are carefully chosen, and preparation techniques have been perfected through many years of practice. Studies indicated that Mangifera indica possesses antidiabetic, anti-oxidant, anti-viral, cardiotonic, hypotensive, and anti-inflammatory properties. Mango contains Mangiferin, a polyphenolic antioxidant and a glucosyl xanthone, has strong antioxidant, anti lipid peroxidation, immunomodulation, cardiotonic, hypotensive, wound healing, antidegenerative and antidiabetic activities.

A novel transdermal drug delivery system: drug-loaded ROS-responsive ferrocene fibers for effective photoprotective and wound healing activity

The present study proposes an innovative transdermal drug delivery system using ferrocene-incorporated fibers to enhance the bioavailability and therapeutic efficacy of ascorbyl tetraisopalmitate. Using electrospinning technology, the authors created ferrocene polymer fibers capable of highly efficient drug encapsulation and controlled release in response to reactive oxygen species commonly found in wound sites. The approach improves upon previous methods significantly by offering higher drug loading capacities and sustained release, directly targeting diseased cells. The results confirm the potential of ferrocene fibers for localized drug delivery, potentially reducing side effects and increasing patient convenience. The method could facilitate the application of bioactive compounds in medical textiles and targeted therapy.

Wound Healing Effectiveness of Berberis baluchistanica and Daphne oleoides Extracts Native to Pakistan

Traditionally in Turkish medicines, Berberis baluchistanica and Daphne oleoides plant are used for the treatment of rheumatism, fever, lumbago, gonorrhea, boils, snake bite antidote, piles, hemorrhoids, as laxative, ulcers and wound healing. The study objective was based on the scientific investigation of traditional claim of wound healing potential of both these wild plants extract in healthy inbreed male albino rats. The wound healing activity of both the extracts was evaluated by adopting excision and incision wound models with a topical dose of 100 mg/kg b.w /day. Wound healing process effectiveness was assessed by measuring wound contraction and epithelization period, measurement of tensile strength of healing skin and histopathological studies of granulating tissue. D. oleoides roots crude methanolic extract (CME) encouraged pronounced wound repairing process in both adopted wound models. In this case, boosted wound retrenchment rate (P &lt; 0.001), shrinkage of epithelialization period (10.13 ± 0.11), high skin disintegration strength (410.0 ± 1.8) and remarkable enhanced granulation tissue weight (P &lt; 0.001) were experienced in albino rats. As well as the granulation tissue histological examination exhibited more collagen with reduced inflammatory cells number propagation versus control. In B. baluchistanica stems CME treated animals, wound contraction, and granulation tissue content were not much prevailed being (P &lt; 0.05) moderately high. The histology of the granulation tissue disclosed greater number of inflamed cells with less collagen than the D. oleoides treated animals. However, it was more amplified compared to control group. The collective research data emphasized that D. oleoides possesses significant wound management activity versus B. baluchistanica, furthermore both extracts rationalize the traditional claim to cure wounds and can be utilized in different wound healing therapies in human beings as well.

Ethnomedicinal Uses, Phytochemistry, Pharmacology, and Toxicology of Ruellia tuberosa L.: A Review.

Natural Products continue to be the purest source of physiologically active molecules employed in the identification of possible lead compounds in the drug discovery process. Acanthaceae is a big plant family with around 2500 species, found primarily in subtropical and tropical regions, as well as the Mediterranean, Australia, and the United States. Several species of the Acanthaceae family have been used traditionally to treat a variety of diseases, including gastrointestinal and cardiovascular ailments, etc. Ruellia tuberosa commonly known as "Mexican Bluebell" is a perennial herb that originated in Central America and has spread to some countries in the Southern tropics and Southeast Asia. It has been utilized as a traditional Rasayana plant from ancient times. R. tuberosa extracts and phytochemicals showed potent bioactivities, such as anticancer, anti-inflammatory, wound healing, antifungal, antimicrobial, anti-diabetic, hypoglycemic, hypolipidemic, gastro-protective, and anthelminthic activities. Chemical analyses have unveiled a range of bioactive constituents within the plant, including alkaloids, flavonoids, saponins, and phenolic compounds, suggestive of its therapeutic potential. Collectively, this review provides an overview of R. tuberosa, encompassing its traditional uses, ethnomedicinal importance, phytochemistry, pharmacological properties, and toxicity.

Evaluation of Arabic gum antibacterial and wound healing activities against Staphylococcus aureus isolates obtained from skin infections

Evaluation of Arabic gum antibacterial and wound healing activities against Staphylococcus aureus isolates obtained from skin infections

Current Status and Applications of Gamma Radiation-induced Graft Copolymerized Chitosan

Chitosan (CS) is a natural polymer obtained by removing acetyl groups from chitin through alkaline hydrolysis. It possesses biodegradable properties and exhibits immunological, antibacterial, and wound-healing activities. This polysaccharide has undergone modification through radiation-induced graft copolymerization to broaden its application scope. The potential applications of CS can be expanded by introducing side chains through grafting. This article aims to review the innovative alternatives of gamma-graft-copolymerized CS and, for the first time, comprehensively examines the current applications of CS derivatives in dye removal, metal adsorption, antibacterial interventions, biomedical practices, drug delivery systems, and tissue engineering.